Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | A multi-biosensor based on immobilized Photosystem II on screen-printed electrodes for the detection of herbicides in river water. |
| Abstract | A multi-biosensor for detection of herbicides and pollutants was constructed using various photosynthetic preparations as biosensing elements. The photosynthetic thylakoid from Spinacia oleracea L., Senecio vulgaris and its mutant resistant to atrazine were immobilized with (BSA-GA) on the surface of screen-printed sensors composed of a graphite-working electrode and Ag/AgCl reference electrode deposited on a polymeric substrate. The biosensor was composed of four flow cells with independent illumination of 650 nm to activate electron transfer in Photosystem II. The principle of the detection was based on the fact that herbicides selectively block electron transport activity in a concentration-dependent manner and that the four PSII biomediators show differential recognition activity toward herbicides. Changes of the activity were registered amperometrically as rate of photoreduction of the artificial electron acceptor DQ. The setup resulted in a reusable herbicide multibiosensor with a good stability (half-life of 16.7 h for spinach thylakoids) and limit of detection of about 10-8Mfor herbicides recovered in spring in river. |
| Source | Biosensors & bioelectronics |
| Keywords | biosensorphotosystemimmobilisationherbicides |
| Journal | Biosensors & bioelectronics |
| Editor | Elsevier Science Publishers., Barking, Regno Unito |
| Year | 2005 |
| Type | Articolo in rivista |
| DOI | 10.1016/j.bios.2004.08.035 |
| Authors | E. Touloupakis, L. Giannoudi, S.A. Piletsky, L. Guzzella, F. Pozzoni, M.T. Giardi |
| Text | 391908 2005 10.1016/j.bios.2004.08.035 biosensor photosystem immobilisation herbicides A multi biosensor based on immobilized Photosystem II on screen printed electrodes for the detection of herbicides in river water. E. Touloupakis, L. Giannoudi, S.A. Piletsky, L. Guzzella, F. Pozzoni, M.T. Giardi Institute of Crystallography Section of IC Bari , National Research Council of Italy CNR , Institute of Bioscience and Technology, Cranfield University, IRSA CNR A multi biosensor for detection of herbicides and pollutants was constructed using various photosynthetic preparations as biosensing elements. The photosynthetic thylakoid from Spinacia oleracea L., Senecio vulgaris and its mutant resistant to atrazine were immobilized with BSA GA on the surface of screen printed sensors composed of a graphite working electrode and Ag/AgCl reference electrode deposited on a polymeric substrate. The biosensor was composed of four flow cells with independent illumination of 650 nm to activate electron transfer in Photosystem II. The principle of the detection was based on the fact that herbicides selectively block electron transport activity in a concentration dependent manner and that the four PSII biomediators show differential recognition activity toward herbicides. Changes of the activity were registered amperometrically as rate of photoreduction of the artificial electron acceptor DQ. The setup resulted in a reusable herbicide multibiosensor with a good stability half life of 16.7 h for spinach thylakoids and limit of detection of about 10 8Mfor herbicides recovered in spring in river. Published version Articolo in rivista Elsevier Science Publishers. 0956 5663 Biosensors bioelectronics Biosensors bioelectronics Biosens. bioelectron. Biosensors bioelectronics. Biosensors and bioelectronics POZZONI FIORENZO mariateresa.giardi GIARDI MARIA TERESA liciamaria.guzzella GUZZELLA LICIA MARIA eleftherios.touloupakis TOULOUPAKIS ELEFTHERIOS |