Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | Purification of phycocyanin from Arthrospira platensis by hydrophobic interaction membrane chromatography |
| Abstract | The cyanobacterium Arthrospira platensis is an important source of phycocyanin, which has many commercial applications in foods, cosmetics and pharmaceutics. In this study, hydrophobic interaction membrane chromatography (HIMC) was used for the first time to separate phycocyanin from allophycocyanin and obtain analytical grade phycocyanin, avoiding packed bed (i.e., packed column) chromatography steps. The extraction of phycobiliproteins was carried out on freeze-dried biomass samples suspended in NaCl 0.1 M, and the phycobiliprotein crude extract was obtained by centrifuging the suspension to eliminate cell debris. The crude extract, after the addition of an appropriate amount of ammonium sulphate, was loaded on an ammonium sulphate responsive commercial hydrophilic polyvinylidene fluoride (PVDF) membrane for the HIMC purification process. Applying a two-step HIMC purification procedure, a phycocyanin purity ratio of 4.20 and a yield of 67.0% was attained. |
| Source | Algal Research 35–333 |
| Keywords | SpirulinaPhycocyaninAllophycocyaninPurificationMembrane chromatography |
| Journal | Algal Research |
| Editor | Elsevier, Netherlands, |
| Year | 2018 |
| Type | Articolo in rivista |
| DOI | 10.1016/j.algal.2018.09.003 |
| Authors | Lauceri R., Zittelli G.C., Maserti B., Torzillo G. |
| Text | 391281 2018 10.1016/j.algal.2018.09.003 ISI Web of Science WOS 000447187700035 Scopus 2 s2.0 85053111290 Spirulina Phycocyanin Allophycocyanin Purification Membrane chromatography Purification of phycocyanin from Arthrospira platensis by hydrophobic interaction membrane chromatography Lauceri R., Zittelli G.C., Maserti B., Torzillo G. Autori CNR The cyanobacterium Arthrospira platensis is an important source of phycocyanin, which has many commercial applications in foods, cosmetics and pharmaceutics. In this study, hydrophobic interaction membrane chromatography HIMC was used for the first time to separate phycocyanin from allophycocyanin and obtain analytical grade phycocyanin, avoiding packed bed i.e., packed column chromatography steps. The extraction of phycobiliproteins was carried out on freeze dried biomass samples suspended in NaCl 0.1 M, and the phycobiliprotein crude extract was obtained by centrifuging the suspension to eliminate cell debris. The crude extract, after the addition of an appropriate amount of ammonium sulphate, was loaded on an ammonium sulphate responsive commercial hydrophilic polyvinylidene fluoride PVDF membrane for the HIMC purification process. Applying a two step HIMC purification procedure, a phycocyanin purity ratio of 4.20 and a yield of 67.0% was attained. 35 Published version https //doi.org/10.1016/j.algal.2018.09.003 Lauceri et al AR 2018 1_s2_0_S2211926418305034_main.pdf Articolo in rivista Elsevier 2211 9264 Algal Research Algal Research rosaria.lauceri LAUCERI ROSARIA graziella.chinizittelli CHINI ZITTELLI GRAZIELLA giuseppe.torzillo TORZILLO GIUSEPPE biancaelena.maserti MASERTI BIANCAELENA |