| Title | Barcoding of Chrysomelidae of Euro-Mediterranean area: efficiency and problematic species |
| Abstract | Leaf beetles (Coleoptera: Chrysomelidae), with more than 37,000 species worldwide and about 2,300 in the Euro-Mediterranean region, are an ecological and economical relevant family, making their molecular identification of interest also in agriculture. This study, part of the Mediterranean Chrysomelidae Barcoding project (www.c-bar.org), aims to: (i) develop a reference Cytochrome c oxidase I (COI) library for the molecular identification of the Euro-Mediterranean Chrysomelidae; (ii) test the efficiency of DNA barcoding for leaf beetles identification; (iii) develop and compare optimal thresholds for distance-based identifications estimated at family and subfamily level, minimizing false positives and false negatives. Within this study, 889 COI nucleotide sequences of 261 species were provided; after the inclusion of information from other sources, a dataset of 7,237 sequences (542 species) was analysed. The average intra-interspecific distances were in the range of those recorded for Coleoptera: 1.6-24%. The estimated barcoding efficiency (~94%) confirmed the usefulness of this tool for Chrysomelidae identification. The few cases of failure were recorded for closely related species (e.g., Cryptocephalus marginellus superspecies, Cryptocephalus violaceus - Cryptocephalus duplicatus and some Altica species), even with morphologically different species sharing the same COI haplotype. Different optimal thresholds were achieved for the tested taxonomic levels, confirming that group-specific thresholds significantly improve molecular identifications. |
| Source | Scientific reports (Nature Publishing Group) 8 (13398) |
| Keywords | coleoptera |
| Journal | Scientific reports (Nature Publishing Group) |
| Editor | Nature Publishing Group, London, Regno Unito |
| Year | 2018 |
| Type | Articolo in rivista |
| DOI | 10.1038/s41598-018-31545-9 |
| Authors | Giulia Magoga, Didem Coral Sahin, Diego Fontaneto, Matteo Montagna |
| Text | 391170 2018 10.1038/s41598 018 31545 9 Scopus 2 s2.0 85052991454 ISI Web of Science WOS 000444022800002 coleoptera Barcoding of Chrysomelidae of Euro Mediterranean area efficiency and problematic species Giulia Magoga, Didem Coral Sahin, Diego Fontaneto, Matteo Montagna Dipartimento di Scienze Agrarie e Ambientali Universita degli Studi di Milano, Via Celoria 2, 20133, Milano, Italy; Consiglio Nazionale delle Ricerche Istituto per lo Studio degli Ecosistemi, Largo Tonolli 50, 28922, Verbania, Italy Leaf beetles Coleoptera Chrysomelidae , with more than 37,000 species worldwide and about 2,300 in the Euro Mediterranean region, are an ecological and economical relevant family, making their molecular identification of interest also in agriculture. This study, part of the Mediterranean Chrysomelidae Barcoding project www.c bar.org , aims to i develop a reference Cytochrome c oxidase I COI library for the molecular identification of the Euro Mediterranean Chrysomelidae; ii test the efficiency of DNA barcoding for leaf beetles identification; iii develop and compare optimal thresholds for distance based identifications estimated at family and subfamily level, minimizing false positives and false negatives. Within this study, 889 COI nucleotide sequences of 261 species were provided; after the inclusion of information from other sources, a dataset of 7,237 sequences 542 species was analysed. The average intra interspecific distances were in the range of those recorded for Coleoptera 1.6 24%. The estimated barcoding efficiency 94% confirmed the usefulness of this tool for Chrysomelidae identification. The few cases of failure were recorded for closely related species e.g., Cryptocephalus marginellus superspecies, Cryptocephalus violaceus Cryptocephalus duplicatus and some Altica species , even with morphologically different species sharing the same COI haplotype. Different optimal thresholds were achieved for the tested taxonomic levels, confirming that group specific thresholds significantly improve molecular identifications. 8 Published version https //www.nature.com/articles/s41598 018 31545 9 magoga 2018_SciRep_Magoga et al.pdf Articolo in rivista Nature Publishing Group 2045 2322 Scientific reports Nature Publishing Group Scientific reports Nature Publishing Group Scientific reports Nature Publishing Group diego.fontaneto FONTANETO DIEGO |
