Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | Assessment of the natural microbial community in a cattle manure-amended soil in presence/absence of the antibiotic sulfamethoxazole |
| Abstract | Livestock and aquaculture activities involve the use of antibiotics. The use of manure and digestate in agricul-tural practices can introduce antibiotics and promote resistant bacteria into terrestrial and aquatic environ-ments. Sulfamethoxazole (SMX) is one of the most commonly prescribed and consumed sulfonamide antibiotic for its ability to inhibit both Gram-positive and Gram-negative bacteria. However, current knowledge about its persistence and effects on natural microbial communities are quite scarce. We investigated in microcosm laboratory experiments the effects on the structure and functioning of a soil natural microbial community treat-ed with cattle manure in presence/absence of the antibiotic SMX (20 mg/kg). At fixed times (0, 7, 13, 20, 60 days) microbiological abundance, viability, activity and SMX residual concentrations (ASE extraction and HPLC-UV detection) were analysed. Moreover, the microbial community diversity was investigated with NGS analyses. The results showed that although SMX had an initial acute negative effect on the cell abundance and viability, the antibiotic was then degraded in just a few days. The microbial community composition was influenced by the different treatments and the effect on different bacterial groups will be shown |
| Source | 3rd Conference on Ecology of Soil Microorganisms, Helsinki, 17-21 Giugno 2018 |
| Keywords | Pharmaceutical degradationsoil microbial communityNGS analyses |
| Year | 2018 |
| Type | Abstract in atti di convegno |
| Authors | Rauseo J, Cardoni M, Patrolecco L, Ademollo N, Di Lenola M, Pescatore T, Spataro F, Barra Caracciolo A, Grenni P |
| Text | 389436 2018 Pharmaceutical degradation soil microbial community NGS analyses Assessment of the natural microbial community in a cattle manure amended soil in presence/absence of the antibiotic sulfamethoxazole Rauseo J, Cardoni M, Patrolecco L, Ademollo N, Di Lenola M, Pescatore T, Spataro F, Barra Caracciolo A, Grenni P irsa CNR 3rd Conference on Ecology of Soil Microorganisms 17 21 June 2018, Helsinki, Finland, Natural resources and bioeconomy studies 29/2018. Abstract Book K Kettunen, O Kiikkila 978 952 326 583 7 Published version 3rd Conference on Ecology of Soil Microorganisms Helsinki 17 21 Giugno 2018 Internazionale Contributo Livestock and aquaculture activities involve the use of antibiotics. The use of manure and digestate in agricul tural practices can introduce antibiotics and promote resistant bacteria into terrestrial and aquatic environ ments. Sulfamethoxazole SMX is one of the most commonly prescribed and consumed sulfonamide antibiotic for its ability to inhibit both Gram positive and Gram negative bacteria. However, current knowledge about its persistence and effects on natural microbial communities are quite scarce. We investigated in microcosm laboratory experiments the effects on the structure and functioning of a soil natural microbial community treat ed with cattle manure in presence/absence of the antibiotic SMX 20 mg/kg . At fixed times 0, 7, 13, 20, 60 days microbiological abundance, viability, activity and SMX residual concentrations ASE extraction and HPLC UV detection were analysed. Moreover, the microbial community diversity was investigated with NGS analyses. The results showed that although SMX had an initial acute negative effect on the cell abundance and viability, the antibiotic was then degraded in just a few days. The microbial community composition was influenced by the different treatments and the effect on different bacterial groups will be shown Abstract Book AbstractBook_luke luobio_29_2018.pdf Abstract in atti di convegno francesca.spataro SPATARO FRANCESCA jasmin.rauseo RAUSEO JASMIN CARDONI MARTINA DI LENOLA MARTINA tanitapescatore PESCATORE TANITA luisa.patrolecco PATROLECCO LUISA anna.barracaracciolo BARRA CARACCIOLO ANNA paola.grenni GRENNI PAOLA nicoletta.ademollo ADEMOLLO NICOLETTA TA.P04.005.011 Vulnerabilita degli ecosistemi delle acque sotterranee e attenuazione naturale degli inquinanti nel suolo e nel sottosuolo |