Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | Substrate stress response of microbial consortia in a MBR for wastewater treatment as detected through a metaproteomic approach |
| Abstract | A lab-scale Membrane BioReactor (MBR) for the treatment of synthetic municipal wastewater was set-up. The functional response of the activated sludge of the plant to a change in the supply of the influent wastewater was investigated. After 60 days at the steady state, the volumetric loading rate (VLR) was suddenly reduced to 65% by reducing the influent flowrate and establishing stress conditions for the biomass. The VLR decrease resulted in a reduction of volatile suspended solids (VSS) in the mixed liquor and in a slight worsening of the chemical oxygen demand (COD) degradation with respect to the previous period. Respirometry results revealed that the autotrophic bacteria more promptly adapted to the organic load perturbation than the heterotrophic ones. More details were provided by the application of metaproteomics. Particularly, several species expressed the same molecular chaperone in different experimental stages, evidencing diverse organic supply stress thresholds by different bacteria. More, coherently with the respirometric approach, some proteins belonging to species based on nitrogen metabolism were found in all samples, confirming the good resistance of autotrophic genera such as Nitrosomonas or Nitrospira to the stress due to VLR decrease. Finally, the expression of 1-butanol dehydrogenase by Thauera butanivorans at the end of the experimentation indicates that some species developed different metabolic pathways such as the degradation of butane, which had not been detected in previous stages. |
| Source | Microbial stress: from Systems to Molecules and Back, Kinsale, Co. Cork, Ireland, 23-25/04/2018 |
| Keywords | StressMetaproteomicsMBRmicrobial community |
| Year | 2018 |
| Type | Poster |
| Authors | Carlo Salerno, Giovanni Berardi, Alfieri Pollice |
| Text | 387313 2018 Stress Metaproteomics MBR microbial community Substrate stress response of microbial consortia in a MBR for wastewater treatment as detected through a metaproteomic approach Carlo Salerno, Giovanni Berardi, Alfieri Pollice IRSA CNR A lab scale Membrane BioReactor MBR for the treatment of synthetic municipal wastewater was set up. The functional response of the activated sludge of the plant to a change in the supply of the influent wastewater was investigated. After 60 days at the steady state, the volumetric loading rate VLR was suddenly reduced to 65% by reducing the influent flowrate and establishing stress conditions for the biomass. The VLR decrease resulted in a reduction of volatile suspended solids VSS in the mixed liquor and in a slight worsening of the chemical oxygen demand COD degradation with respect to the previous period. Respirometry results revealed that the autotrophic bacteria more promptly adapted to the organic load perturbation than the heterotrophic ones. More details were provided by the application of metaproteomics. Particularly, several species expressed the same molecular chaperone in different experimental stages, evidencing diverse organic supply stress thresholds by different bacteria. More, coherently with the respirometric approach, some proteins belonging to species based on nitrogen metabolism were found in all samples, confirming the good resistance of autotrophic genera such as Nitrosomonas or Nitrospira to the stress due to VLR decrease. Finally, the expression of 1 butanol dehydrogenase by Thauera butanivorans at the end of the experimentation indicates that some species developed different metabolic pathways such as the degradation of butane, which had not been detected in previous stages. Postprint Microbial stress from Systems to Molecules and Back Kinsale, Co. Cork, Ireland 23 25/04/2018 Internazionale Contributo Poster alfieri.pollice POLLICE ALFIERI giovanni.berardi BERARDI GIOVANNI carlo.salerno SALERNO CARLO |