| Abstract | Microbial biofilms play a key role in substrate colonization in aquatic environments, as hot spots of biodiversity and sources of secondary metabolites with biotechnological potential. In Terra Nova Bay (Ross Sea, Antarctica), microbial biofilm communities are under-explored in their structure and functions; particularly, their response to environmental and/or anthropogenic forcings remains to be elucidated yet. Funded by National Antarctic Research Program (XXXIII Italian expedition), the ANT-Biofilm project "Microbial colonization of benthic ANTarctic environments: response of microbial abundances, diversity, activities and larval settlement to natural or anthropogenic disturbances and search for secondary metabolites" (PNRA16_00105) includes short and long term (3-12 months) experiments in two bays (Road Bay and Tethys Bay) characterized by anthropogenic (i.e. sewage) and natural (i.e. low salinity) stressors, respectively. Total, viable and respiring prokaryotes, culturable bacteria, potential enzymatic activity rates, physiological community profiles, microalgae and macrobenthic community are studied. Selected biofilm samples will be sequenced and analyzed by hybridization (i.e. Card-FISH) and bacterial isolates screened for antibiotic resistance and metabolite production. Physical-chemical environmental characterization will be performed. This contribution reports the preliminary results obtained by the four Research Units during the short term experiments. |
| Authors | Caruso G., Azzaro M., Dell'Acqua O., Lo Giudice A., Fazi S., Caroppo C., Azzaro F., La Ferla R., Maimone G., Laganà P., Marinelli F., Berini F., Marcone G.L., Pichon G., Chiantore M. (the ANT-BIOFILM research group) |
| Text | 387221 2018 biofilm Microbes Antarctica Substrate colonization in the Ross Sea, Antarctica the ANT BIOFILM Project Caruso G., Azzaro M., Dell Acqua O., Lo Giudice A., Fazi S., Caroppo C., Azzaro F., La Ferla R., Maimone G., Lagana P., Marinelli F., Berini F., Marcone G.L., Pichon G., Chiantore M. the ANT BIOFILM research group Institute for Coastal Marine Environment IAMC , National Research Council, Messina; Department of Earth, Environment and Life Sciences DISTAV , University of Genoa; Department of Chemical, Biological, Pharmaceutical and Environmental Sciences CHIBIOFARM , University of Messina; Water Research Institute IRSA , National Research Council; Institute for Coastal Marine Environment IAMC , National Research Council, Taranto; Department of Biomedical and Dental Sciences and Morphofunctional Imaging. University of Messina; Department of Biotechnology and Life Sciences DBSV , University of Insubria, Varese; Barbier Group, Ste Sigolene, France Microbial biofilms play a key role in substrate colonization in aquatic environments, as hot spots of biodiversity and sources of secondary metabolites with biotechnological potential. In Terra Nova Bay Ross Sea, Antarctica , microbial biofilm communities are under explored in their structure and functions; particularly, their response to environmental and/or anthropogenic forcings remains to be elucidated yet. Funded by National Antarctic Research Program XXXIII Italian expedition , the ANT Biofilm project Microbial colonization of benthic ANTarctic environments response of microbial abundances, diversity, activities and larval settlement to natural or anthropogenic disturbances and search for secondary metabolites PNRA16_00105 includes short and long term 3 12 months experiments in two bays Road Bay and Tethys Bay characterized by anthropogenic i.e. sewage and natural i.e. low salinity stressors, respectively. Total, viable and respiring prokaryotes, culturable bacteria, potential enzymatic activity rates, physiological community profiles, microalgae and macrobenthic community are studied. Selected biofilm samples will be sequenced and analyzed by hybridization i.e. Card FISH and bacterial isolates screened for antibiotic resistance and metabolite production. Physical chemical environmental characterization will be performed. This contribution reports the preliminary results obtained by the four Research Units during the short term experiments. Preprint POLAR 2018 Davos Switzerland 15/06/2018 26/06/2018 Internazionale Contributo Poster gabriella.caruso CARUSO GABRIELLA filippo.azzaro AZZARO FILIPPO carmela.caroppo CAROPPO CARMELA maurizio.azzaro AZZARO MAURIZIO stefano.fazi FAZI STEFANO rosabruna.laferla LA FERLA ROSABRUNA angelina.logiudice LO GIUDICE ANGELINA giovanna.maimone MAIMONE GIOVANNA DTA.AD001.103.011 PNRA BANDO 2016_A1_16_00105 RESP. G. CARUSO CNR IAMC DTA.AD002.343.001 Colonizzazione microbica di ambienti bentonici in Antartide risposte di abbondanze, diversita e attivita microbiche ed insediamento larvale a disturbi naturali o antropici e ricerca di metaboliti secondari Programma Nazionale di Ricerca in Antarti |
