| Title | Enterococcus faecium ST17 from Coastal Marine Sediment Carrying Transferable Multidrug Resistance Plasmids |
| Abstract | The multidrug-resistant Enterococcus faecium 17i48, sequence type 17, from marine sediment, carrying erm(B), tet(M), and tet(L) genes, was analyzed for the presence of antibiotic resistance plasmids and for the ability to transfer resistance genes. The strain was found to harbor the replicon type (repA) of pRE25, pRUM, pHTβ, and the axe-txe toxin-antitoxin (TA) system. In mating experiments, tet(M) and tet(L) were cotransferred with the repApRE25, whereas erm(B) was consistently cotransferred with the axe-txe and repApRUM, suggesting that tetracycline and erythromycin resistance genes were carried on different elements both transferable by conjugation, likely via pHTβ-mediated mobilization. Hybridization and PCR mapping demonstrated that tet(M) and tet(L) were located in tandem on a pDO1-like plasmid that also carried the repApRE25, whereas erm(B) was carried by a pRUM-like plasmid. Sequencing of the latter plasmid showed a high nucleotide identity with pRUM and the presence of cat, aadE, sat4, and a complete aphA resistance genes. These findings show that the genetic features of E. faecium 17i48 are consistent with a hospital-adapted clone and suggest that antibiotic resistance may spread in the environment, also in the absence of antibiotic pressure, due to TA system plasmid maintenance. |
| Source | Microbial drug resistance (Larchmont N.Y.) 22 (7), pp. 523–530 |
| Keywords | antibiotic resistanceantibiotic resistance plasmid characterizationenterococcitoxin/antitoxin system |
| Journal | Microbial drug resistance (Larchmont N.Y.) |
| Editor | Mary Ann Liebert,, Larchmont, NY, Stati Uniti d'America |
| Year | 2016 |
| Type | Articolo in rivista |
| DOI | 10.1089/mdr.2015.0222 |
| Authors | Morroni G.; Di Cesare A.; Di Sante L.; Brenciani A.; Vignaroli C.; Pasquaroli S.; Giovanetti E.; Sabatino R.; Rossi L.; Magnani M.; Biavasco F. |
| Text | 386464 2016 10.1089/mdr.2015.0222 Scopus 2 s2.0 84990927896 antibiotic resistance antibiotic resistance plasmid characterization enterococci toxin/antitoxin system Enterococcus faecium ST17 from Coastal Marine Sediment Carrying Transferable Multidrug Resistance Plasmids Morroni G.; Di Cesare A.; Di Sante L.; Brenciani A.; Vignaroli C.; Pasquaroli S.; Giovanetti E.; Sabatino R.; Rossi L.; Magnani M.; Biavasco F. Department of Biomedical Sciences and Public Health, Polytechnic University of Marche, Ancona, , Italy; Department of Life and Environmental Sciences, Polytechnic University of Marche, Ancona, , Italy; Biochemistry and Molecular Biology Section, Department of Biomolecular Sciences, University of Urbino Carlo Bo, Urbino, , Italy The multidrug resistant Enterococcus faecium 17i48, sequence type 17, from marine sediment, carrying erm B , tet M , and tet L genes, was analyzed for the presence of antibiotic resistance plasmids and for the ability to transfer resistance genes. The strain was found to harbor the replicon type repA of pRE25, pRUM, pHTβ, and the axe txe toxin antitoxin TA system. In mating experiments, tet M and tet L were cotransferred with the repApRE25, whereas erm B was consistently cotransferred with the axe txe and repApRUM, suggesting that tetracycline and erythromycin resistance genes were carried on different elements both transferable by conjugation, likely via pHTβ mediated mobilization. Hybridization and PCR mapping demonstrated that tet M and tet L were located in tandem on a pDO1 like plasmid that also carried the repApRE25, whereas erm B was carried by a pRUM like plasmid. Sequencing of the latter plasmid showed a high nucleotide identity with pRUM and the presence of cat, aadE, sat4, and a complete aphA resistance genes. These findings show that the genetic features of E. faecium 17i48 are consistent with a hospital adapted clone and suggest that antibiotic resistance may spread in the environment, also in the absence of antibiotic pressure, due to TA system plasmid maintenance. 22 Published version http //www.scopus.com/inward/record.url eid=2 s2.0 84990927896 partnerID=q2rCbXpz Articolo 2016_A0_088.pdf Articolo in rivista Mary Ann Liebert, 1076 6294 Microbial drug resistance Larchmont N.Y. Microbial drug resistance Larchmont N.Y. Microb. drug resist. Larchmont N.Y. Microbial drug resistance Larchmont N.Y. MDR Larchmont N.Y. andrea.dicesare DI CESARE ANDREA |
