| Abstract | Type II Toxin-Antitoxin systems (TAs), known for their presence in virulent and antibiotic resistant bacterial strains, were recently identified in Salmonella enterica isolates. However, the relationships between the presence of TAs (ccdAB and vapBC) and the epidemiological and genetic features of different non-typhoidal Salmonella serovars are largely unknown, reducing our understanding of the ecological success of different serovars. Salmonella enterica isolates from different sources, belonging to different serovars and epidemiologically unrelated according to ERIC profiles, were investigated for the presence of type II TAs, plasmid content, and antibiotic resistance. The results showed the ubiquitous presence of the vapBC gene in all the investigated Salmonella isolates, but a diverse distribution of ccdAB, which was detected in the most widespread Salmonella serovars, only. Analysis of the plasmid toxin ccdB translated sequence of four selected Salmonella isolates showed the presence of the amino acid substitution R99W, known to impede in vitro the lethal effect of CcdB toxin in the absence of its cognate antitoxin CcdA. These findings suggest a direct role of the TAs in promoting adaptability and persistence of the most prevalent Salmonella serovars, thus implying a wider eco-physiological role for these type II TAs. |
| Text | 386387 2016 10.1038/srep28759 Scopus 2 s2.0 84977118038 Microbial ecology microbiology Diverse distribution of Toxin Antitoxin II systems in Salmonella enterica serovars Di Cesare A.; Losasso C.; Barco L.; Eckert E.M.; Conficoni D.; Sarasini G.; Corno G.; Ricci A. Microbial Ecology Group, National Research Council, Institute of Ecosystem Study CNR ISE , Largo Tonolli 50, Verbania, 28822, , Italy; Food Safety Department, Istituto Zooprofilattico Sperimentale delle Venezie, viale dell Universita 10, Legnaro, 35020, , Italy; Department Animal Medicine, Production and Health, University of Padua, viale dell Universita, Legnaro, 35020, , Italy Type II Toxin Antitoxin systems TAs , known for their presence in virulent and antibiotic resistant bacterial strains, were recently identified in Salmonella enterica isolates. However, the relationships between the presence of TAs ccdAB and vapBC and the epidemiological and genetic features of different non typhoidal Salmonella serovars are largely unknown, reducing our understanding of the ecological success of different serovars. Salmonella enterica isolates from different sources, belonging to different serovars and epidemiologically unrelated according to ERIC profiles, were investigated for the presence of type II TAs, plasmid content, and antibiotic resistance. The results showed the ubiquitous presence of the vapBC gene in all the investigated Salmonella isolates, but a diverse distribution of ccdAB, which was detected in the most widespread Salmonella serovars, only. Analysis of the plasmid toxin ccdB translated sequence of four selected Salmonella isolates showed the presence of the amino acid substitution R99W, known to impede in vitro the lethal effect of CcdB toxin in the absence of its cognate antitoxin CcdA. These findings suggest a direct role of the TAs in promoting adaptability and persistence of the most prevalent Salmonella serovars, thus implying a wider eco physiological role for these type II TAs. 6 Published version http //www.scopus.com/inward/record.url eid=2 s2.0 84977118038 partnerID=q2rCbXpz Articolo 2016_A0_062.pdf Articolo in rivista Nature Publishing Group 2045 2322 Scientific reports Nature Publishing Group Scientific reports Nature Publishing Group Scientific reports Nature Publishing Group estermaria.eckert ECKERT ESTER MARIA gianluca.corno CORNO GIANLUCA andrea.dicesare DI CESARE ANDREA |
