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DatoValore
TitleMicroplastics increase int1 abundance and persistence of wastewater-derived microbes in freshwater
AbstractPlastic pollution is a major global concern with several million microplastic particles entering every day freshwater ecosystems via wastewater discharge, together with human pathogens. Microplastic particles stimulate biofilm formation (plastisphere) throughout the water column and have the potential to affect microbial community structure, especially enhancing the proliferation of biohazardous bacteria in pelagic systems. To test this scenario, we simulated the inflow of treated wastewater into a temperate lake using a continuous culture system with a gradient of concentration of microplastic particles. We followed the effect of microplastics on the microbial community structure by ARISA and on the occurrence of integrase 1 (int1, by qPCR), a marker associated to mobile genetic elements known as a proxy for anthropic effects on the spread of antimicrobial resistance genes.
SourceSIM 2017 XLV Congresso Nazionale della Società Italiana di Microbiologia, Genova, 27-30 settembre 2017
KeywordsMicroplastics; Antibiotic Resistance; Wastewater-derived microbes; Freshwaters
Year2017
TypePoster
AuthorsDi Cesare A., Eckert E. M., Kettner M. T., Arias-Andrés M., Fontaneto D., Grossart H., Corno G.
Text385368 2017 Microplastics; Antibiotic Resistance; Wastewater derived microbes; Freshwaters Microplastics increase int1 abundance and persistence of wastewater derived microbes in freshwater Di Cesare A., Eckert E. M., Kettner M. T., Arias Andres M., Fontaneto D., Grossart H., Corno G. CNR ISE; Dept. Experimental Limnology Leibniz Institute of Freshwater Ecology and Inland Fisheries Stechlin Germany ; Plastic pollution is a major global concern with several million microplastic particles entering every day freshwater ecosystems via wastewater discharge, together with human pathogens. Microplastic particles stimulate biofilm formation plastisphere throughout the water column and have the potential to affect microbial community structure, especially enhancing the proliferation of biohazardous bacteria in pelagic systems. To test this scenario, we simulated the inflow of treated wastewater into a temperate lake using a continuous culture system with a gradient of concentration of microplastic particles. We followed the effect of microplastics on the microbial community structure by ARISA and on the occurrence of integrase 1 int1, by qPCR , a marker associated to mobile genetic elements known as a proxy for anthropic effects on the spread of antimicrobial resistance genes. Published version SIM 2017 XLV Congresso Nazionale della Societa Italiana di Microbiologia Genova 27 30 settembre 2017 Internazionale Contributo Poster 2017_POster_SIM.pdf Poster estermaria.eckert ECKERT ESTER MARIA gianluca.corno CORNO GIANLUCA diego.fontaneto FONTANETO DIEGO andrea.dicesare DI CESARE ANDREA