Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | Mitonuclear discordance as a confounding factor in the DNA taxonomy of monogonont rotifers |
| Abstract | Discordance between mitochondrial and nuclear phylogenies is being increasingly recognized in animals and may confound DNA-based taxonomy. This is especially relevant for taxa whose microscopic size often challenges any effort to distinguish between cryptic species without the assistance of molecular data. Regarding mitonuclear discordance, two strikingly contrasting scenarios have been recently demonstrated in the monogonont rotifers of the genus Brachionus. While strict mitonuclear concordance was observed in the marine B. plicatilis species complex, widespread hybridization-driven mitonuclear discordance was revealed in the freshwater B. calyciflorus species complex. Here, we investigated the frequency of occurrence and the potential drivers of mitonuclear discordance in three additional freshwater monogonont rotifer taxa, and assessed its potential impact on the reliability of DNA taxonomy results based on commonly used single markers. We studied the cryptic species complexes of Keratella cochlearis, Polyarthra dolichoptera and Synchaeta pectinata. Phylogenetic reconstructions were based on the mitochondrial barcoding marker cytochrome c oxidase subunit I gene and the nuclear internal transcribed spacer 1 locus, which currently represent the two most typical genetic markers used in rotifer DNA taxonomy. Species were delimited according to each marker separately using a combination of tree-based coalescent, distance-based and allele-sharing-based approaches. Mitonuclear discordance was observed in all species complexes with incomplete lineage sorting and unresolved phylogenetic reconstructions recognized as the likely drivers. Evidence from additional sources, such as morphology and ecology, is thus advisable for deciding between often contrasting mitochondrial and nuclear species scenarios in these organisms. |
| Source | Zoologica scripta 47, pp. 122–132 |
| Keywords | rotifera |
| Journal | Zoologica scripta |
| Editor | Blackwell, Oxford, Regno Unito |
| Year | 2018 |
| Type | Articolo in rivista |
| DOI | 10.1111/zsc.12264 |
| Authors | Obertegger U., Cieplinski A., Fontaneto D. & Papakostas S., |
| Text | 381586 2018 10.1111/zsc.12264 Scopus 2 s2.0 85039165099 ISI Web of Science WOS 000426596900011 rotifera Mitonuclear discordance as a confounding factor in the DNA taxonomy of monogonont rotifers Obertegger U., Cieplinski A., Fontaneto D. Papakostas S., Fondazione Mach Trento, Italy; CNR ISE Verbania, Italy; Turku University, Finland Discordance between mitochondrial and nuclear phylogenies is being increasingly recognized in animals and may confound DNA based taxonomy. This is especially relevant for taxa whose microscopic size often challenges any effort to distinguish between cryptic species without the assistance of molecular data. Regarding mitonuclear discordance, two strikingly contrasting scenarios have been recently demonstrated in the monogonont rotifers of the genus Brachionus. While strict mitonuclear concordance was observed in the marine B. plicatilis species complex, widespread hybridization driven mitonuclear discordance was revealed in the freshwater B. calyciflorus species complex. Here, we investigated the frequency of occurrence and the potential drivers of mitonuclear discordance in three additional freshwater monogonont rotifer taxa, and assessed its potential impact on the reliability of DNA taxonomy results based on commonly used single markers. We studied the cryptic species complexes of Keratella cochlearis, Polyarthra dolichoptera and Synchaeta pectinata. Phylogenetic reconstructions were based on the mitochondrial barcoding marker cytochrome c oxidase subunit I gene and the nuclear internal transcribed spacer 1 locus, which currently represent the two most typical genetic markers used in rotifer DNA taxonomy. Species were delimited according to each marker separately using a combination of tree based coalescent, distance based and allele sharing based approaches. Mitonuclear discordance was observed in all species complexes with incomplete lineage sorting and unresolved phylogenetic reconstructions recognized as the likely drivers. Evidence from additional sources, such as morphology and ecology, is thus advisable for deciding between often contrasting mitochondrial and nuclear species scenarios in these organisms. 47 Published version http //onlinelibrary.wiley.com/doi/10.1111/zsc.12264/full obe 2018_ZoolScr_Obertegger et al.pdf Articolo in rivista Blackwell 0300 3256 Zoologica scripta Zoologica scripta Zool. scr. Zoologica scripta. diego.fontaneto FONTANETO DIEGO |