| Title | High-throughput sequencing revealed novel Dehalococcoidia in dechlorinating microbial enrichments from PCB-contaminated marine sediments. |
| Abstract | In this study, six PCE-to-ethene dechlorinating cultures, fed with a fermentable substrate (lactate) or hydrogen as electron donor, were obtained from PCB and PCE dechlorinating microcosms constructed with PCB-contaminated marine sediments. A novel Chloroflexi member (OTU-DIS1) affiliated to Dehalococcoidales Incertae Sedis, only distantly related to known dechlorinating bacteria, dominated the enrichment cultures (up to 86% of total OTUs). Sulfate-, thiosulfate- and sulfur-reducing bacteria affiliated to genera Desulfobacter, Dethiosulfatibacter and Desulfuromusa were also found to lesser extent. Remarkably, tceA, vcrA and the bifunctional PCE/PCB dehalogenase genes pcbA1, pcbA4 and pcbA5 were found in all dechlorinating microbial enrichments indicating the coexistence of different Dehalococcoides mccartyi strains. The reductive dechlorination rate in each culture remained unvaried over long-term operation ( 30 months) and ranged between 0.85 and 0.97 mmol Cl-1 released L-1 d-1 in the lactate-fed microbial enrichments and between 0.66 and 0.85 mmol Cl-1 released L-1 d-1 in the H2-fed microbial enrichments. Overall, this study highlights the presence of yet unexplored biodiversity in PCBs contaminated marine sediments and indicates these environments as promising sources of novel organohalide-respiring bacteria. |
| Source | FEMS microbiology, ecology (print) 93 (11) |
| Keywords | bioremediationnext generation sequencing |
| Journal | FEMS microbiology, ecology (print) |
| Editor | Elsevier, Amsterdam, Paesi Bassi |
| Year | 2017 |
| Type | Articolo in rivista |
| DOI | 10.1093/femsec/fix134 |
| Authors | Matturro, Bruna; Frascadore, Emanuela; Rossetti, Simona |
| Text | 379101 2017 10.1093/femsec/fix134 ISI Web of Science WOS 29040506 bioremediation next generation sequencing High throughput sequencing revealed novel Dehalococcoidia in dechlorinating microbial enrichments from PCB contaminated marine sediments. Matturro, Bruna; Frascadore, Emanuela; Rossetti, Simona Water Research Institute, IRSA CNR, Via Salaria km 29,300, 00015 Monterotondo RM , Italy. In this study, six PCE to ethene dechlorinating cultures, fed with a fermentable substrate lactate or hydrogen as electron donor, were obtained from PCB and PCE dechlorinating microcosms constructed with PCB contaminated marine sediments. A novel Chloroflexi member OTU DIS1 affiliated to Dehalococcoidales Incertae Sedis, only distantly related to known dechlorinating bacteria, dominated the enrichment cultures up to 86% of total OTUs . Sulfate , thiosulfate and sulfur reducing bacteria affiliated to genera Desulfobacter, Dethiosulfatibacter and Desulfuromusa were also found to lesser extent. Remarkably, tceA, vcrA and the bifunctional PCE/PCB dehalogenase genes pcbA1, pcbA4 and pcbA5 were found in all dechlorinating microbial enrichments indicating the coexistence of different Dehalococcoides mccartyi strains. The reductive dechlorination rate in each culture remained unvaried over long term operation 30 months and ranged between 0.85 and 0.97 mmol Cl 1 released L 1 d 1 in the lactate fed microbial enrichments and between 0.66 and 0.85 mmol Cl 1 released L 1 d 1 in the H2 fed microbial enrichments. Overall, this study highlights the presence of yet unexplored biodiversity in PCBs contaminated marine sediments and indicates these environments as promising sources of novel organohalide respiring bacteria. 93 Published version Articolo in rivista Elsevier 0168 6496 FEMS microbiology, ecology print FEMS microbiology, ecology print FEMS microbiol. ecol. print FEMS microbiology, ecology. print Federation of European Microbiological Societies microbiology, ecology print simona.rossetti ROSSETTI SIMONA bruna.matturro MATTURRO BRUNA |
