| Title | Phage-host associations in a full-scale activated sludge plant during sludge bulking |
| Abstract | Sludge bulking, a notorious microbial issue in activated sludge plants, is always accompanied by dramatic changes in the bacterial community. Despite large numbers of phages in sludge systems, their responses to sludge bulking and phage-host associations during bulking are unknown. In this study, high-throughput sequencing of viral metagenomes and bacterial 16S rRNA genes were employed to characterize viral and bacterial communities in a sludge plant under different sludge conditions (sludge volume index (SVI) of 180, 132, and 73 ml/g). Bulking sludges (SVI > 125 ml/g) taken about 10 months apart exhibited similar bacterial and viral composition. This reflects ecological resilience of the sludge microbial community and indicates that changes in viral and bacterial populations correlate closely with each other. Overgrowth of "Candidatus Microthrix parvicella" led to filamentous bulking, but few corresponding viral genotypes were identified. In contrast, sludge viromes were characterized by numerous contigs associated with "Candidatus Accumulibacter phosphatis," suggesting an abundance of corresponding phages in the sludge viral community. Notably, while nitrifiers (mainly Nitrosomonadaceae and Nitrospiraceae) declined significantly along with sludge bulking, their corresponding viral contigs were identified more frequently and with greater abundance in the bulking viromes, implying that phage-mediated lysis might contribute to the loss of autotrophic nitrifiers under bulking conditions. |
| Source | Applied microbiology and biotechnology 101 (16), pp. 6495–6504 |
| Keywords | 16S rRNA gene pyrosequencingPhage-host associationSludge bulkingViral metagenome analysis |
| Journal | Applied microbiology and biotechnology |
| Editor | Springer, Heidelberg ;, Germania |
| Year | 2017 |
| Type | Articolo in rivista |
| DOI | 10.1007/s00253-017-8429-8 |
| Authors | Liu, Ruyin; Liu, Ruyin; Qi, Rong; Wang, Juan; Zhang, Yu; Zhang, Yu; Liu, Xinchun; Liu, Xinchun; Rossetti, Simona; Tandoi, Valter; Yang, Min; Yang, Min |
| Text | 379096 2017 10.1007/s00253 017 8429 8 Scopus 2 s2.0 85026452732 16S rRNA gene pyrosequencing Phage host association Sludge bulking Viral metagenome analysis Phage host associations in a full scale activated sludge plant during sludge bulking Liu, Ruyin; Liu, Ruyin; Qi, Rong; Wang, Juan; Zhang, Yu; Zhang, Yu; Liu, Xinchun; Liu, Xinchun; Rossetti, Simona; Tandoi, Valter; Yang, Min; Yang, Min Chinese Academy of Sciences; Research Center for Eco Environmental Sciences Chinese Academy of Sciences; Consiglio Nazionale delle Ricerche Sludge bulking, a notorious microbial issue in activated sludge plants, is always accompanied by dramatic changes in the bacterial community. Despite large numbers of phages in sludge systems, their responses to sludge bulking and phage host associations during bulking are unknown. In this study, high throughput sequencing of viral metagenomes and bacterial 16S rRNA genes were employed to characterize viral and bacterial communities in a sludge plant under different sludge conditions sludge volume index SVI of 180, 132, and 73 ml/g . Bulking sludges SVI > 125 ml/g taken about 10 months apart exhibited similar bacterial and viral composition. This reflects ecological resilience of the sludge microbial community and indicates that changes in viral and bacterial populations correlate closely with each other. Overgrowth of Candidatus Microthrix parvicella led to filamentous bulking, but few corresponding viral genotypes were identified. In contrast, sludge viromes were characterized by numerous contigs associated with Candidatus Accumulibacter phosphatis, suggesting an abundance of corresponding phages in the sludge viral community. Notably, while nitrifiers mainly Nitrosomonadaceae and Nitrospiraceae declined significantly along with sludge bulking, their corresponding viral contigs were identified more frequently and with greater abundance in the bulking viromes, implying that phage mediated lysis might contribute to the loss of autotrophic nitrifiers under bulking conditions. 101 Published version http //www.scopus.com/record/display.url eid=2 s2.0 85026452732 origin=inward Articolo in rivista Springer 0175 7598 Applied microbiology and biotechnology Applied microbiology and biotechnology Appl. microbiol. biotechnol. Applied microbiology and biotechnology. Applied microbiology and biotechnology Print simona.rossetti ROSSETTI SIMONA |
