Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | Unravelling the core microbiome of biofilms in cooling tower systems |
| Abstract | In this study, next generation sequencing and catalyzed reporter deposition fluorescence in situ hybridization, combined with confocal microscopy, were used to provide insights into the biodiversity and structure of biofilms collected from four full-scale European cooling systems. Water samples were also analyzed to evaluate the impact of suspended microbes on biofilm formation. A common core microbiome, containing members of the families Sphingomonadaceae, Comamonadaceae and Hyphomicrobiaceae, was found in all four biofilms, despite the water of each coming from different sources (river and groundwater). This suggests that selection of the pioneer community was influenced by abiotic factors (temperature, pH) and tolerances to biocides. Members of the Sphingomonadaceae were assumed to play a key role in initial biofilm formation. Subsequent biofilm development was driven primarily by light availability, since biofilms were dominated by phototrophs in the two studied 'open' systems. Their interactions with other microbial populations then shaped the structure of the mature biofilm communities analyzed. |
| Source | Biofouling (Online) |
| Keywords | Biofilms; NGS; cooling systems; core microbiome; pioneer species |
| Journal | Biofouling (Online) |
| Editor | Gordon and Breach Publishing Group., Amsterdam, Paesi Bassi |
| Year | 2017 |
| Type | Articolo in rivista |
| DOI | 10.1080/08927014.2017.1367386 |
| Authors | Di Gregorio L., Tandoi V., Congestri R., Rossetti S., Di Pippo F. |
| Text | 379095 2017 10.1080/08927014.2017.1367386 ISI Web of Science WOS WOS 000423098600002 Scopus 2 s2.0 85031099395 Biofilms; NGS; cooling systems; core microbiome; pioneer species Unravelling the core microbiome of biofilms in cooling tower systems Di Gregorio L., Tandoi V., Congestri R., Rossetti S., Di Pippo F. Istituto di Ricerca Sulle Acque, IRSA CNR In this study, next generation sequencing and catalyzed reporter deposition fluorescence in situ hybridization, combined with confocal microscopy, were used to provide insights into the biodiversity and structure of biofilms collected from four full scale European cooling systems. Water samples were also analyzed to evaluate the impact of suspended microbes on biofilm formation. A common core microbiome, containing members of the families Sphingomonadaceae, Comamonadaceae and Hyphomicrobiaceae, was found in all four biofilms, despite the water of each coming from different sources river and groundwater . This suggests that selection of the pioneer community was influenced by abiotic factors temperature, pH and tolerances to biocides. Members of the Sphingomonadaceae were assumed to play a key role in initial biofilm formation. Subsequent biofilm development was driven primarily by light availability, since biofilms were dominated by phototrophs in the two studied open systems. Their interactions with other microbial populations then shaped the structure of the mature biofilm communities analyzed. Published version https //www.scopus.com/record/display.uri eid=2 s2.0 85031099395 origin=resultslist sort=plf f src=s st1=Di Pippo st2=Francesca nlo=1 nlr=20 nls=afprfnm t sid=52bc6f949f35709c6c38b67f63c68c88 sot=anl sdt=aut sl=40 s=AU ID%28%22Di Pippo%2c Francesca%22 15831265700%29 relpos=7 citeCnt=11 searchTerm= Unravelling the core microbiome of biofilms in cooling tower systems Unravelling the core microbiome of biofilms in cooling tower systems.pdf Articolo in rivista Gordon and Breach Publishing Group. 1029 2454 Biofouling Online Biofouling Online Biofouling Online valter.tandoi TANDOI VALTER simona.rossetti ROSSETTI SIMONA francesca.dipippo DI PIPPO FRANCESCA |