| Title | Syntrophic acetate oxidation during the two-phase anaerobic digestion of waste activated sludge: Microbial population, Gibbs free energy and kinetic modelling |
| Abstract | A system using a two-phase anaerobic configuration (mesophilic/thermophilic) was tested by feeding waste activated sludge (WAS). The first acidogenic stage presented a hydraulic retention time (HRT) of 3 days, while the second methanogenic stage had an HRT of 10 days. Both raw and ultrasonically pretreated WAS samples were utilized for the experiment. Previous Fluorescence in Situ Hybridization (FISH) observations, revealed that in the thermophilic phase, the acetoclastic methanogenesis was likely replaced by a nonacetoclastic pathway, namely, syntrophic acetate oxidation (SAO). A modified version of Anaerobic Digestion Model n°1 (ADM1), accounting for the SAO pathway, was implemented and calibrated. The proposed model addressed the relationship between the hydrogen concentration and Gibbs free energy and showed the thermodynamic feasibility of the SAO pathway, while simultaneously highlighting the role played by hydrogenotrophic methanogens in maintaining a sufficiently low hydrogen partial pressure so that the SAO was energetically feasible. The estimated energy loss was estimated to be approximately 20% due to the switch of the microbial pathway from acetoclastic methanogenesis to SAO. |
| Source | International biodeterioration & biodegradation 125, pp. 177–188 |
| Keywords | waste activated sludgesyntrophic acetate oxidationenergy assessmentdual phase AD |
| Journal | International biodeterioration & biodegradation |
| Editor | Elsevier Applied Science,, Barking, Regno Unito |
| Year | 2017 |
| Type | Articolo in rivista |
| DOI | 10.1016/j.ibiod.2017.09.017 |
| Authors | Montecchio D.; Esposito G.; Gagliano M.C.; Gallipoli A.; Gianico A.; Braguglia C.M. |
| Text | 379080 2017 10.1016/j.ibiod.2017.09.017 Scopus 2 s2.0 85030106756 waste activated sludge syntrophic acetate oxidation energy assessment dual phase AD Syntrophic acetate oxidation during the two phase anaerobic digestion of waste activated sludge Microbial population, Gibbs free energy and kinetic modelling Montecchio D.; Esposito G.; Gagliano M.C.; Gallipoli A.; Gianico A.; Braguglia C.M. Water Research Institute IRSA CNR, Area della Ricerca RM1, Via Salaria km. 29,300, Monterotondo, Roma, 00015, , Italy; University of Cassino and the Southern Lazio, Department of Civil and Mechanical Engineering, Via Di Biasio, 43, Cassino, FR, 03043, , Italy A system using a two phase anaerobic configuration mesophilic/thermophilic was tested by feeding waste activated sludge WAS . The first acidogenic stage presented a hydraulic retention time HRT of 3 days, while the second methanogenic stage had an HRT of 10 days. Both raw and ultrasonically pretreated WAS samples were utilized for the experiment. Previous Fluorescence in Situ Hybridization FISH observations, revealed that in the thermophilic phase, the acetoclastic methanogenesis was likely replaced by a nonacetoclastic pathway, namely, syntrophic acetate oxidation SAO . A modified version of Anaerobic Digestion Model n°1 ADM1 , accounting for the SAO pathway, was implemented and calibrated. The proposed model addressed the relationship between the hydrogen concentration and Gibbs free energy and showed the thermodynamic feasibility of the SAO pathway, while simultaneously highlighting the role played by hydrogenotrophic methanogens in maintaining a sufficiently low hydrogen partial pressure so that the SAO was energetically feasible. The estimated energy loss was estimated to be approximately 20% due to the switch of the microbial pathway from acetoclastic methanogenesis to SAO. 125 Published version http //www.scopus.com/record/display.url eid=2 s2.0 85030106756 origin=inward Syntrophic acetate oxidation during the two phase anaerobic digestion of waste activated sludge Microbial population, Gibbs free energy and kinetic modelling Montecchio et al., 2017 syntrophic acetate ox .pdf Articolo in rivista Elsevier Applied Science, 0964 8305 International biodeterioration biodegradation International biodeterioration biodegradation Int. biodeterior. biodegrad. International biodeterioration and biodegradation. camillamaria.braguglia BRAGUGLIA CAMILLA MARIA andrea.gianico GIANICO ANDREA daniele.montecchio MONTECCHIO DANIELE agata.gallipoli GALLIPOLI AGATA TA.P07.001.005 Recupero di risorse da rifiuti e biomasse |
