| Title | Biotreatment and bioassessment of heavy metal removal by sulphate reducing bacteria in fixed bed reactors |
| Abstract | In this work a batch-optimised mixture (w/w %: 6% leaves, 9% compost, 3% Fe(0), 30% silica sand, 30% perlite, 22% limestone) was investigated in a continuous fixed bed column reactor for the treatment of synthetic acid-mine drainage (AMD). A column reactor was inoculated with sulphate-reducing bacteria and fed with a solution containing sulphate and heavy metals (As(V), Cd, Cr(VI), Cu and Zn). At steady state, sulphate abatement was 50 ± 10%, while metals were totally removed. A degradation rate constant (k) of 0.015 ± 0.001 h-1 for sulphate removal was determined from column data by assuming a first order degradation rate. Reduction of AMD toxicity was assessed by using the nematode Caenorhabditis elegans as a test organism. A lethality assay was performed with the toxicants before and after the treatment, showing that only 5% of the animals were still alive after 48 h in presence of the contaminants, while the percentage increased to 73% when the nematodes were exposed to the solution eluted from the column. |
| Source | Water research (Oxf.) 44 (1), pp. 151–158 |
| Keywords | BioassessmentBiotreatmentCaenorhabditis elegansFixed bed reactorsHeavy metalsSulphate reducing bacteria |
| Journal | Water research (Oxf.) |
| Editor | Pergamon Press., New York, Regno Unito |
| Year | 2010 |
| Type | Articolo in rivista |
| DOI | 10.1016/j.watres.2009.09.013 |
| Authors | Cruz Viggi, C.; Pagnanelli, F.; Cibati, A.; Uccelletti, D.; Palleschi, C.; Toro, L. |
| Text | 378960 2010 10.1016/j.watres.2009.09.013 Scopus 2 s2.0 71549170197 Bioassessment Biotreatment Caenorhabditis elegans Fixed bed reactors Heavy metals Sulphate reducing bacteria Biotreatment and bioassessment of heavy metal removal by sulphate reducing bacteria in fixed bed reactors Cruz Viggi, C.; Pagnanelli, F.; Cibati, A.; Uccelletti, D.; Palleschi, C.; Toro, L. Universita degli Studi di Roma La Sapienza; Universita degli Studi di Roma La Sapienza In this work a batch optimised mixture w/w % 6% leaves, 9% compost, 3% Fe 0 , 30% silica sand, 30% perlite, 22% limestone was investigated in a continuous fixed bed column reactor for the treatment of synthetic acid mine drainage AMD . A column reactor was inoculated with sulphate reducing bacteria and fed with a solution containing sulphate and heavy metals As V , Cd, Cr VI , Cu and Zn . At steady state, sulphate abatement was 50 ± 10%, while metals were totally removed. A degradation rate constant k of 0.015 ± 0.001 h 1 for sulphate removal was determined from column data by assuming a first order degradation rate. Reduction of AMD toxicity was assessed by using the nematode Caenorhabditis elegans as a test organism. A lethality assay was performed with the toxicants before and after the treatment, showing that only 5% of the animals were still alive after 48 h in presence of the contaminants, while the percentage increased to 73% when the nematodes were exposed to the solution eluted from the column. 44 Published version http //www.scopus.com/record/display.url eid=2 s2.0 71549170197 origin=inward Articolo in rivista Pergamon Press. 0043 1354 Water research Oxf. Water research Oxf. Water res. Oxf. Water research. Oxf. carolina.cruzviggi CRUZ VIGGI CAROLINA |
