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DatoValore
TitleMetabolic analysis of the removal of formic acid by unacclimated activated sludge
AbstractThis paper investigates the removal of formic acid by unacclimated biomass from a municipal activated sludge wastewater treatment plant. The biomass was initially able to remove formic acid, but its removal rate and Oxygen Uptake Rate (OUR) decreased with time, until formic acid removal stopped before the formic acid had been exhausted. Formaldehyde was removed in a similar way, whereas the same biomass was simultaneously able to grow and store PHAs when acetic acid was used as substrate.Batch tests with glycine and 13C NMR analysis were performed, showing that unacclimated biomass was not able to synthesize all the metabolic intermediates from formic acid alone. At least glycine needed to be externally supplemented, in order to activate the serine synthesis pathway. A small amount of formic acid removal in the absence of growth was also possible through formaldehyde formation and its further conversion to formalin (1,2-formaldehyde dimer), whereas no PHAs were formed.
SourceWater research (Oxf.) 44 (11), pp. 3393–3400
KeywordsActivated sludgeFormic acidNMR, C, isotopomer analysis 13
JournalWater research (Oxf.)
EditorPergamon Press., New York, Regno Unito
Year2010
TypeArticolo in rivista
DOI10.1016/j.watres.2010.03.032
AuthorsCruz Viggi, Carolina; Dionisi, Davide; Miccheli, Alfredo; Valerio, Mariacristina; Majone, Mauro
Text378958 2010 10.1016/j.watres.2010.03.032 Scopus 2 s2.0 77953028701 Activated sludge Formic acid NMR, C, isotopomer analysis 13 Metabolic analysis of the removal of formic acid by unacclimated activated sludge Cruz Viggi, Carolina; Dionisi, Davide; Miccheli, Alfredo; Valerio, Mariacristina; Majone, Mauro Universita degli Studi di Roma La Sapienza; Istituto Nazionale Tumori Regina Elena This paper investigates the removal of formic acid by unacclimated biomass from a municipal activated sludge wastewater treatment plant. The biomass was initially able to remove formic acid, but its removal rate and Oxygen Uptake Rate OUR decreased with time, until formic acid removal stopped before the formic acid had been exhausted. Formaldehyde was removed in a similar way, whereas the same biomass was simultaneously able to grow and store PHAs when acetic acid was used as substrate.Batch tests with glycine and 13C NMR analysis were performed, showing that unacclimated biomass was not able to synthesize all the metabolic intermediates from formic acid alone. At least glycine needed to be externally supplemented, in order to activate the serine synthesis pathway. A small amount of formic acid removal in the absence of growth was also possible through formaldehyde formation and its further conversion to formalin 1,2 formaldehyde dimer , whereas no PHAs were formed. 44 Published version http //www.scopus.com/record/display.url eid=2 s2.0 77953028701 origin=inward Articolo in rivista Pergamon Press. 0043 1354 Water research Oxf. Water research Oxf. Water res. Oxf. Water research. Oxf. carolina.cruzviggi CRUZ VIGGI CAROLINA