Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | Comparison of two molecular methods to assess soil microbial diversity |
| Keywords | Denaturing Gradient Gel Electrophoresis-DGGEIn Situ Hybridization-FISHBacterial 16S rRNAPhyto-assisted bioremediationPolychlorinated biphenyls (PCBs) |
| Year | 2017 |
| Type | Contributo in volume |
| DOI | 10.1007/978-3-319-63336-7 |
| Authors | Di Lenola M, Grenni P, Proença DN, Morais PV, Barra Caracciolo A |
| Text | 376969 2017 10.1007/978 3 319 63336 7 Denaturing Gradient Gel Electrophoresis DGGE In Situ Hybridization FISH Bacterial 16S rRNA Phyto assisted bioremediation Polychlorinated biphenyls PCBs Comparison of two molecular methods to assess soil microbial diversity Di Lenola M, Grenni P, Proença DN, Morais PV, Barra Caracciolo A Di Lenola, Grenni, Barra Caracciolo IRSA CNR Proença, Morais CEMMPRE University of Coimbra, Rua Luis Reis Santos, 3030 788 Coimbra, Portugal Our knowledge of microbial soil biodiversity depends on the ability to combine different observation levels, ranging from the phenotypic to the molecular one, including direct visualization of microorganisms by using an epifluorescence microscope. Soil microbial communities, although still largely undiscovered, represent one of the biggest part of present biodiversity and play a key role in all soil processes. For example, microbial abundance, activity and composition largely determine the sustainable productivity of agricultural land or the degradation of organic pollutants. The diversity of microbial communities associated with plant roots is enormous, in the order of thousands of species. The simultaneous use of different molecular methods makes it possible to have a more holistic knowledge of the soil microbial community and to assess changes in it under different conditions. In this chapter we report the application to the same soil samples of two different phylogenetic molecular techniques, i.e. DGGE Denaturing Gradient Gel Electrophoresis and FISH Fluorescence In Situ Hybridization . DGGE is a useful fingerprint technique of the overall microbial community based on amplification of 16S rRNA. The FISH technique identify, without extracting nucleic acids, active microbial cells at different phylogenetic levels from domains to species under an epifluorescence microscope. An example of the application of both methodologies to assessing the microbial community composition of soil samples from a phyto assisted bioremediation experiment of a contaminated soil by polychlorinated biphenyls PCBs is reported. Soil Biological Communities and Ecosystem Resilience Martin Lukac, Paola Grenni, Mauro Gamboni 978 3 319 63335 0 Published version https //link.springer.com/book/10.1007%2F978 3 319 63336 7 Series Sustainability in Plant and Crop Protection Progetto Europeo COST Action BioLink Linking belowground biodiversity and ecosystem function in European forests COST Action number FP1305, European Cooperation in Science and Technology Capitolo libro pubblicato Book.pdf Contributo in volume DI LENOLA MARTINA anna.barracaracciolo BARRA CARACCIOLO ANNA paola.grenni GRENNI PAOLA TA.P04.005.011 Vulnerabilita degli ecosistemi delle acque sotterranee e attenuazione naturale degli inquinanti nel suolo e nel sottosuolo |