| Title | Coupling a bio-accumulator organism and MALDI-TOF MS: an early warning detection system for microcystins in water bodies. |
| Abstract | Abstract Microcystins produced by freshwater cyanobacteria pose serious threats to human health and are a growing problem in drinking water supplies worldwide. Toxin detection and identification in water reservoirs when cyanobacterial density is still low is a key step to predict harmful algal blooms and to allow the safe use of the resource. For this purpose, developing sensitive and rapid methods of analysis is crucial. Adopting a strategy that couples the use of a filter-feeder organism (Unio elongatulus, Pfeiffer 1825 (Bivalvia: Unionidae)) with matrix assisted laser desorption ionization--time of flight mass spectrometry (MALDI-TOF MS) technology, effective microcystin detection was achieved. The performances of MALDI-TOF MS, high-liquid-performance chromatography with diode array detector (HPLC-DAD) and enzyme-linked immunosorbent assays (ELISA) test were compared. The results obtained
in this study suggest that the exploitation of an efficient bioaccumulator organism, coupled with a rapid and sensitive analytical
method, can be a very useful strategy in monitoring programs for early and prompt risk management. |
| Source | Journal of applied phycology (Dordr., Online) |
| Keywords | Microcystin detection . Sentinel organism . Unio elongatulus .MALDI-TOF MS . Early warning system |
| Journal | Journal of applied phycology (Dordr., Online) |
| Editor | Kluwer, Dordrecht, Paesi Bassi |
| Year | 2017 |
| Type | Articolo in rivista |
| DOI | 10.1007/s10811-017-1193-4 |
| Authors | Rosaria Lauceri (1), Martina Austoni (1), Francesca Caviglia(2), Lyudmila Kamburska(1), Andrea Lami(1), Giuseppe Morabito(1), Valentin Pflueger(3), Ana Margarita Silva Benavides(4), Mauro Tonolla(5), Giuseppe Torzillo(6), Nicoletta Riccardi(1) |
| Text | 374398 2017 10.1007/s10811 017 1193 4 Microcystin detection . Sentinel organism . Unio elongatulus .MALDI TOF MS . Early warning system Coupling a bio accumulator organism and MALDI TOF MS an early warning detection system for microcystins in water bodies. Rosaria Lauceri 1 , Martina Austoni 1 , Francesca Caviglia 2 , Lyudmila Kamburska 1 , Andrea Lami 1 , Giuseppe Morabito 1 , Valentin Pflueger 3 , Ana Margarita Silva Benavides 4 , Mauro Tonolla 5 , Giuseppe Torzillo 6 , Nicoletta Riccardi 1 1 National Research Council, Institute of Ecosystem Study, Verbania, Italy; 2 Piedmontese Regional Agency for the Environmental Prevention and Protection, Omegna, Italy; 3 Mabritec AG, Riehen, Switzerland; 4 Escuela de Biologia, Centro de Investigacion en Ciencias del Mar y Limnologia CIMAR , Universidad de Costa Rica, San Pedro, San Jose 11501, Costa Rica; 5 Laboratory of Applied Microbiology, University of Applied Sciences and Arts of Southern Switzerland, Bellinzona, Switzerland; 6 National Research Council, Institute of Ecosystem Study, Florence, Italy Abstract Microcystins produced by freshwater cyanobacteria pose serious threats to human health and are a growing problem in drinking water supplies worldwide. Toxin detection and identification in water reservoirs when cyanobacterial density is still low is a key step to predict harmful algal blooms and to allow the safe use of the resource. For this purpose, developing sensitive and rapid methods of analysis is crucial. Adopting a strategy that couples the use of a filter feeder organism Unio elongatulus, Pfeiffer 1825 Bivalvia Unionidae with matrix assisted laser desorption ionization time of flight mass spectrometry MALDI TOF MS technology, effective microcystin detection was achieved. The performances of MALDI TOF MS, high liquid performance chromatography with diode array detector HPLC DAD and enzyme linked immunosorbent assays ELISA test were compared. The results obtained in this study suggest that the exploitation of an efficient bioaccumulator organism, coupled with a rapid and sensitive analytical method, can be a very useful strategy in monitoring programs for early and prompt risk management. Published version https //link.springer.com/article/10.1007/s10811 017 1193 4 Coupling a bio accumulator organism and MALDI TOF MS an early warning detection system for microcystins in water bodies AUTHOR S COPY Lauceri et al._2017.pdf Articolo in rivista Kluwer 1573 5176 Journal of applied phycology Dordr., Online Journal of applied phycology Dordr., Online J. appl. phycol. Dordr., Online Journal of applied phycology. Dordr., Online martina.austoni AUSTONI MARTINA lyudmilatodorova.kamburska KAMBURSKA LYUDMILA TODOROVA giuseppe.morabito MORABITO GIUSEPPE rosaria.lauceri LAUCERI ROSARIA nicolettarita.riccardi RICCARDI NICOLETTA RITA andrea.lami LAMI ANDREA giuseppe.torzillo TORZILLO GIUSEPPE |
