| Title | Highly complex substrates lead to dynamic bacterial community for polyhydroxyalkanoates production. |
| Abstract | Mixed microbial cultures (MMC) and waste/surplus substrates, as hardwood spent sulfite liquor, are being used to decrease polyhydroxyalkanoates' (PHA) production costs. The process involves two or three steps, being the selection step a crucial one. For the industrial implementation of this strategy, reactor stability in terms of both performance and microbial community presence has to be considered. A long-term operation of a sequencing batch reactor under feast/famine conditions was performed along with microbial community identification/quantification using FISH and DGGE. The community was found to be extremely dynamic, dominated by Alphaproteobacteria, with Paracoccus and Rhodobacter present, both PHA-storing microorganisms. 16S rRNA gene clone library further revealed that side populations' non-PHA accumulators were able to strive (Agrobacterium, Flavobacteria, and Brachymonas). Nevertheless, reactor performance in terms of PHA storage was stable during operation time. The monitoring of the MMC population evolution provided information on the relation between community structure and process operation. |
| Source | Journal of industrial microbiology and biotechnology (Online) |
| Keywords | Bacterial community dynamics; Hardwood spent sulfite liquor (HSSL); Mixed microbial cultures (MMC); Molecular methods; Polyhydroxyalkanoates (PHA) |
| Journal | Journal of industrial microbiology and biotechnology (Online) |
| Editor | Springer, Berlin, Germania |
| Year | 2017 |
| Type | Articolo in rivista |
| DOI | 10.1007/s10295-017-1951-y |
| Authors | Queiros, Diogo; Fonseca, Alexandre; Rossetti, Simona; Serafim, Luisa S; Lemos, Paulo C |
| Text | 372380 2017 10.1007/s10295 017 1951 y ISI Web of Science WOS 28497282 Bacterial community dynamics; Hardwood spent sulfite liquor HSSL ; Mixed microbial cultures MMC ; Molecular methods; Polyhydroxyalkanoates PHA Highly complex substrates lead to dynamic bacterial community for polyhydroxyalkanoates production. Queiros, Diogo; Fonseca, Alexandre; Rossetti, Simona; Serafim, Luisa S; Lemos, Paulo C Chemistry Department, CICECO Aveiro Institute of Materials, University of Aveiro, Campus Universitario de Santiago, 3810 193, Aveiro, Portugal. Mixed microbial cultures MMC and waste/surplus substrates, as hardwood spent sulfite liquor, are being used to decrease polyhydroxyalkanoates PHA production costs. The process involves two or three steps, being the selection step a crucial one. For the industrial implementation of this strategy, reactor stability in terms of both performance and microbial community presence has to be considered. A long term operation of a sequencing batch reactor under feast/famine conditions was performed along with microbial community identification/quantification using FISH and DGGE. The community was found to be extremely dynamic, dominated by Alphaproteobacteria, with Paracoccus and Rhodobacter present, both PHA storing microorganisms. 16S rRNA gene clone library further revealed that side populations non PHA accumulators were able to strive Agrobacterium, Flavobacteria, and Brachymonas . Nevertheless, reactor performance in terms of PHA storage was stable during operation time. The monitoring of the MMC population evolution provided information on the relation between community structure and process operation. Published version Articolo in rivista Springer 1476 5535 Journal of industrial microbiology and biotechnology Online Journal of industrial microbiology and biotechnology Online J. ind. microbiol. biotechnol. Online Journal of industrial microbiology and biotechnology. Online Journal of industrial microbiology biotechnology Internet Online simona.rossetti ROSSETTI SIMONA |
