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TitleEnhancing a multi-stage process for olive oil mill wastewater valorization towards polyhydroxyalkanoates and biogas production
AbstractOlive oil mill wastewaters (OMW) cause serious environmental pollution problems due to their high level of production and specific chemical characteristics. An innovative disposal treatment and simultaneous valorization technology consists of using OMW as a no-cost substrate for mixed microbial culture (MMC)-based polyhydroxyalkanoates (PHAs) production process. Here, an MMC multi-stage process was developed in which after acidogenic fermentation, OMW was subjected to a solid liquid separation step by centrifugation. The resulting solid fraction was converted into methane through anaerobic digestion in order to recover energy for the process, whereas the liquid fraction was used as feedstock for both the biomass selection and the PHA accumulation step. Most of OMW soluble chemical oxygen demand (COD) content in the liquid fraction (approximately 70%) was removed in a sequencing batch reactor (SBR) obtaining a high PHA production (about 532 mgCOD, in the feast phase). In particular, the strategy to uncouple COD feed from nitrogen supply within the SBR cycle, along with the operation of a settling phase before the famine phase, allowed to enhance the selective pressure towards PHA-storing microorganisms. Polymer production significantly increased in the accumulation reactor up to approximately 2470 mgCOD/L. Finally, anaerobic digestion tests on the OMW solid fraction showed a relevant COD conversion into methane (up to 100%).
SourceChemical engineering journal ( Print) 317, pp. 280–289
KeywordsBiogas generationMixed microbial culture (MMC)Olive oil mill wastewater (OMW)Polyhydroxyalkanoates (PHA)Sequencing batch reactor (SBR)
JournalChemical engineering journal ( Print)
EditorElsevier Sequoia, Lausanne, Svizzera
Year2017
TypeArticolo in rivista
DOI10.1016/j.cej.2017.02.094
AuthorsCampanari, Sabrina; Augelletti, Floriana; Rossetti, Simona; Sciubba, Fabio; Villano, Marianna; Majone, Mauro
Text372360 2017 10.1016/j.cej.2017.02.094 Scopus 2 s2.0 85014081335 ISI Web of Science WOS WOS 000400226700025 Biogas generation Mixed microbial culture MMC Olive oil mill wastewater OMW Polyhydroxyalkanoates PHA Sequencing batch reactor SBR Enhancing a multi stage process for olive oil mill wastewater valorization towards polyhydroxyalkanoates and biogas production Campanari, Sabrina; Augelletti, Floriana; Rossetti, Simona; Sciubba, Fabio; Villano, Marianna; Majone, Mauro Universita degli Studi di Roma La Sapienza; Istituto di Ricerca sulle Acque, Italy Olive oil mill wastewaters OMW cause serious environmental pollution problems due to their high level of production and specific chemical characteristics. An innovative disposal treatment and simultaneous valorization technology consists of using OMW as a no cost substrate for mixed microbial culture MMC based polyhydroxyalkanoates PHAs production process. Here, an MMC multi stage process was developed in which after acidogenic fermentation, OMW was subjected to a solid liquid separation step by centrifugation. The resulting solid fraction was converted into methane through anaerobic digestion in order to recover energy for the process, whereas the liquid fraction was used as feedstock for both the biomass selection and the PHA accumulation step. Most of OMW soluble chemical oxygen demand COD content in the liquid fraction approximately 70% was removed in a sequencing batch reactor SBR obtaining a high PHA production about 532 mgCOD, in the feast phase . In particular, the strategy to uncouple COD feed from nitrogen supply within the SBR cycle, along with the operation of a settling phase before the famine phase, allowed to enhance the selective pressure towards PHA storing microorganisms. Polymer production significantly increased in the accumulation reactor up to approximately 2470 mgCOD/L. Finally, anaerobic digestion tests on the OMW solid fraction showed a relevant COD conversion into methane up to 100% . 317 Published version http //www.scopus.com/record/display.url eid=2 s2.0 85014081335 origin=inward Enhancing a multi stage process for olive oil mill wastewater valorization towards polyhydroxyalkanoates and biogas production Chemical Engineering Journal 317, 280 289, 2017 Campanari et al ChemEngJour2017.pdf Articolo in rivista Elsevier Sequoia 1385 8947 Chemical engineering journal Print Chemical engineering journal Print Chem. eng. j. Print Chemical engineering journal. Print simona.rossetti ROSSETTI SIMONA