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TitleInfluence of the Po River runoff on the bacterioplankton community along trophic and salinity gradients in the Northern Adriatic Sea
AbstractThe Po River runoff strongly affects the oceanographic and ecological characteristics of the Northern Adriatic Sea. Catalysed reported deposition - fluorescence in situ hybridization (CARD-FISH) analysis was employed to assess how the composition of the coastal bacterioplankton community is influenced by the river runoff in two different seasons (spring and autumn). Samples were collected from the water column along a coastal-offshore transect in the Northern Adriatic Sea at different depths. Four clone libraries were then constructed from coastal (0 m) and offshore waters (-65 m). Higher abundances of bacteria were recorded in coastal waters as compared with the offshore samples. This result was mainly due to the trophic state of the water column, and it was related to salinity. Particularly, Actinobacteria and Gammaproteobacteria were affected by the riverine inputs, whereas Bacteroidetes and Alphaproteobacteria showed only minor responses. This was particularly clear in the autumn sample, in which a clear difference between the coastal and the offshore samples was found due to a strong influence of the less saline river water with high nutrient concentrations. Analysis of 205 partial length 16S rRNA gene sequences indicated a high diversity with the dominance of Alphaproteobacteria, Bacteroidetes and Gammaproteobacteria mainly affiliated to coastal and marine bacterioplankton clades. Actinobacteria were also detected and, together with Gammaproteobacteria, strongly follow the freshwater intrusion. Overall, our results indicate that the seasonal difference in the Po River discharge greatly affects the bacterioplankton community. In spring we observed a smooth transition from coastal to open-sea conditions owing to the more superficial freshwater plume. In autumn the deeper freshwater intrusion in the coastal site and thermal stratification offshore resulted in a marked difference between the coastal and offshore microbial communities.
SourceMarine ecology (Berl.) 37 (6), pp. 1386–1397
Keywords16S rRNA Gene sequencingAdriatic Seabacterial community compositionCARD-FISHRITMARE
JournalMarine ecology (Berl.)
EditorBlackwell, Berlin, Germania
Year2016
TypeArticolo in rivista
DOI10.1111/maec.12355
AuthorsPizzetti, Ilaria; Lupini, Giuliano; Aubry, Fabrizio Bernardi; Acri, Francesco; Fuchs, Bernhard M.; Fazi, Stefano
Text372316 2016 10.1111/maec.12355 ISI Web of Science WOS 000390401400017 16S rRNA Gene sequencing Adriatic Sea bacterial community composition CARD FISH RITMARE Influence of the Po River runoff on the bacterioplankton community along trophic and salinity gradients in the Northern Adriatic Sea Pizzetti, Ilaria; Lupini, Giuliano; Aubry, Fabrizio Bernardi; Acri, Francesco; Fuchs, Bernhard M.; Fazi, Stefano CNR; CNR; MPI The Po River runoff strongly affects the oceanographic and ecological characteristics of the Northern Adriatic Sea. Catalysed reported deposition fluorescence in situ hybridization CARD FISH analysis was employed to assess how the composition of the coastal bacterioplankton community is influenced by the river runoff in two different seasons spring and autumn . Samples were collected from the water column along a coastal offshore transect in the Northern Adriatic Sea at different depths. Four clone libraries were then constructed from coastal 0 m and offshore waters 65 m . Higher abundances of bacteria were recorded in coastal waters as compared with the offshore samples. This result was mainly due to the trophic state of the water column, and it was related to salinity. Particularly, Actinobacteria and Gammaproteobacteria were affected by the riverine inputs, whereas Bacteroidetes and Alphaproteobacteria showed only minor responses. This was particularly clear in the autumn sample, in which a clear difference between the coastal and the offshore samples was found due to a strong influence of the less saline river water with high nutrient concentrations. Analysis of 205 partial length 16S rRNA gene sequences indicated a high diversity with the dominance of Alphaproteobacteria, Bacteroidetes and Gammaproteobacteria mainly affiliated to coastal and marine bacterioplankton clades. Actinobacteria were also detected and, together with Gammaproteobacteria, strongly follow the freshwater intrusion. Overall, our results indicate that the seasonal difference in the Po River discharge greatly affects the bacterioplankton community. In spring we observed a smooth transition from coastal to open sea conditions owing to the more superficial freshwater plume. In autumn the deeper freshwater intrusion in the coastal site and thermal stratification offshore resulted in a marked difference between the coastal and offshore microbial communities. 37 Published version Influence of the Po River runoff on the bacterioplankton community along trophic and salinity gradients in the northern Adriatic Sea Pizzetti_et_al 2016 Marine_Ecology with sup1.pdf Articolo in rivista Blackwell 0173 9565 Marine ecology Berl. Marine ecology Berl. Mar. ecol. Berl. Marine ecology. Berl. Pubblicazioni della Stazione Zoologica di Napoli. I, Marine ecology Berl. Marine ecology Berlin. Print Berl. francesco.acri ACRI FRANCESCO fabrizio.bernardiaubry BERNARDI AUBRY FABRIZIO stefano.fazi FAZI STEFANO