Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | Chlamydial seasonal dynamics and isolation of "Candidatus Neptuno chlamydia vexilliferae' from a Tyrrhenian coastal lake |
| Abstract | The Chlamydiae are a phylum of obligate intracellular bacteria comprising important human and animal pathogens, yet their occurrence in the environment, their phylogenetic diversity and their host range has been largely underestimated. We investigated the seasonality of environmental chlamydiae in a Tyrrhenian coastal lake. By catalysed reporter deposition fluorescence in situ hybridization, we quantified the small planktonic cells and detected a peak in the abundance of environmental chlamydiae in early autumn with up to 5.9x10(4) cells ml(-1). Super-resolution microscopy improved the visualization and quantification of these bacteria and enabled the detection of pleomorphic chlamydial cells in their protist host directly in an environmental sample. To isolate environmental chlamydiae together with their host, we applied a high-throughput limited dilution approach and successfully recovered a Vexillifera sp., strain harbouring chlamydiae (93% 16S rRNA sequence identity to Simkania negevensis), tentatively named CandidatusNeptunochlamydia vexilliferae'. Transmission electron microscopy in combination with fluorescence in situ hybridization was used to prove the intracellular location of these bacteria representing the first strain of marine chlamydiae stably maintained alongside with their host in a laboratory culture. Taken together, this study contributes to a better understanding of the distribution and diversity of environmental chlamydiae in previously neglected marine environments. |
| Source | Environmental microbiology (Print) 18 (8), pp. 2405–2417 |
| Keywords | RITMAREChlamydiaeCoastal lakes |
| Journal | Environmental microbiology (Print) |
| Editor | Blackwell Science., Oxford, Regno Unito |
| Year | 2016 |
| Type | Articolo in rivista |
| DOI | 10.1111/1462-2920.13111 |
| Authors | Pizzetti, Ilaria; Schulz, Frederik; Tyml, Tomas; Fuchs, Bernhard M.; Amann, Rudolf; Horn, Matthias; Fazi, Stefano |
| Text | 372289 2016 10.1111/1462 2920.13111 ISI Web of Science WOS 000383388800014 Scopus 2 s2.0 84983329232 RITMARE Chlamydiae Coastal lakes Chlamydial seasonal dynamics and isolation of Candidatus Neptuno chlamydia vexilliferae from a Tyrrhenian coastal lake Pizzetti, Ilaria; Schulz, Frederik; Tyml, Tomas; Fuchs, Bernhard M.; Amann, Rudolf; Horn, Matthias; Fazi, Stefano IRSA CNR; University of Vienna; Univiversity of South Bohemia; Masaryk University; Max Planck Institute for Marine Microbiology The Chlamydiae are a phylum of obligate intracellular bacteria comprising important human and animal pathogens, yet their occurrence in the environment, their phylogenetic diversity and their host range has been largely underestimated. We investigated the seasonality of environmental chlamydiae in a Tyrrhenian coastal lake. By catalysed reporter deposition fluorescence in situ hybridization, we quantified the small planktonic cells and detected a peak in the abundance of environmental chlamydiae in early autumn with up to 5.9x10 4 cells ml 1 . Super resolution microscopy improved the visualization and quantification of these bacteria and enabled the detection of pleomorphic chlamydial cells in their protist host directly in an environmental sample. To isolate environmental chlamydiae together with their host, we applied a high throughput limited dilution approach and successfully recovered a Vexillifera sp., strain harbouring chlamydiae 93% 16S rRNA sequence identity to Simkania negevensis , tentatively named CandidatusNeptunochlamydia vexilliferae . Transmission electron microscopy in combination with fluorescence in situ hybridization was used to prove the intracellular location of these bacteria representing the first strain of marine chlamydiae stably maintained alongside with their host in a laboratory culture. Taken together, this study contributes to a better understanding of the distribution and diversity of environmental chlamydiae in previously neglected marine environments. 18 Published version https //www.scopus.com/record/display.uri eid=2 s2.0 84983329232 origin=inward txGid=754057f8aa4de91d303151340062c0ea La rilevanza di questo lavoro e evidenziata dal fatto che Viana and Buchrieser Institut Pasteur, Paris, France , su invito dell Editor in Chief, scrissero un Highlight dal titolo Environmental treasures co isolation of the first marine Chlamydiae and its protozoan host Environmental Microbiology 2016 18 8 , 2295 2297 al fine di sottolineare l importanza della scoperta di Pizzetti et al., 2016 Environmental Microbiology 18 8 , 2405 2417 . Chlamydial seasonal dynamics and isolation of Candidatus Neptunochlamydia vexilliferae from a Tyrrhenian coastal lake Pizzetti_et_al 2016 Environmental_Microbiology.pdf Highlight Environmental treasures co isolation of the first marine Chlamydiae and its protozoan host Highlight dove si sottolineare l importanza della scoperta pubblicata da Pizzetti et al. 2016 . Viana et al 2016_HIGHLIGHT Pizzetti et al 2016.pdf Articolo in rivista Blackwell Science. 1462 2912 Environmental microbiology Print Environmental microbiology Print Environ. microbiol. Print stefano.fazi FAZI STEFANO |