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DatoValore
TitleBiodegradation potentiality of psychrophilic bacterial strain Oleispira antarctica RB-8(T)
AbstractThe present study is focused on assessing the growth and hydrocarbon-degrading capability of the psychrophilic strain Oleispira antarctica RB-8(T). This study considered six hydrocarbon mixtures that were tested for 22 days at two different cultivation temperatures (4 and 15 degrees C). During the incubation period, six sub-aliquots of each culture at different times were processed for total bacterial abundance and GC-FID (gas chromatography flame ionization detection) hydrocarbon analysis.
SourceMarine pollution bulletin. 105 (1), pp. 125–130
KeywordsBioremediationHydrocarbonoclastic bacteriaOil spillOleispira antarcticaPsychrophilic bacteria
JournalMarine pollution bulletin.
EditorMacmillan,, London, Regno Unito
Year2016
TypeArticolo in rivista
DOI10.1016/j.marpolbul.2016.02.041
AuthorsGentile, G.; Bonsignore, M.; Santisi, S.; Catalfamo, M.; Giuliano, L.; Genovese, L.; Yakimov, M. M.; Denaro, R.; Genovese, M.; Cappello, S.
Text371443 2016 10.1016/j.marpolbul.2016.02.041 ISI Web of Science WOS 000375816000029 Bioremediation Hydrocarbonoclastic bacteria Oil spill Oleispira antarctica Psychrophilic bacteria Biodegradation potentiality of psychrophilic bacterial strain Oleispira antarctica RB 8 T Gentile, G.; Bonsignore, M.; Santisi, S.; Catalfamo, M.; Giuliano, L.; Genovese, L.; Yakimov, M. M.; Denaro, R.; Genovese, M.; Cappello, S. CNR; Univ Messina; CNR The present study is focused on assessing the growth and hydrocarbon degrading capability of the psychrophilic strain Oleispira antarctica RB 8 T . This study considered six hydrocarbon mixtures that were tested for 22 days at two different cultivation temperatures 4 and 15 degrees C . During the incubation period, six sub aliquots of each culture at different times were processed for total bacterial abundance and GC FID gas chromatography flame ionization detection hydrocarbon analysis. Results from DNA extraction and DAPI 4 ,6 diamidino 2 phenylindole staining showed a linear increase during the first 18 days of the experiment in almost all the substrates used; both techniques showed a good match, but the difference in values obtained was approximately one order of magnitude. GC FID results revealed a substantial hydrocarbon degradation rate in almost all hydrocarbon sources and in particular at 15 degrees C rather than 4 degrees C for commercial oil engine, oily waste, fuel jet, and crude oil . A more efficient degradation was observed in cultures grown with diesel and bilge water at 4 degrees C. C 2016 Elsevier Ltd. All rights reserved. 105 Published version Articolo in rivista Macmillan, 0025 326X Marine pollution bulletin. Marine pollution bulletin. Mar. Pollut. Bull. Marine pollution bulletin. lucrezia.genovese GENOVESE LUCREZIA mikhail.iakimov IAKIMOV MIKHAIL renata.denaro DENARO RENATA simone.cappello CAPPELLO SIMONE