Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | Analysis of defence systems and a conjugative IncP-1 plasmid in the marine polyaromatic hydrocarbons-degrading bacterium Cycloclasticus sp 78-ME |
| Abstract | Marine prokaryotes have evolved a broad repertoire of defence systems to protect their genomes from lateral gene transfer including innate or acquired immune systems and infection-induced programmed cell suicide and dormancy. Here we report on the analysis of multiple defence systems present in the genome of the strain Cycloclasticus sp. 78-ME isolated from petroleum deposits of the tanker Amoco Milford Haven'. Cycloclasticus are ubiquitous bacteria globally important in polyaromatic hydrocarbons degradation in marine environments. Two defence islands' were identified in 78-ME genome: the first harbouring CRISPR-Cas with toxin-antitoxin system, while the second was composed by an array of genes for toxin-antitoxin and restriction-modification proteins. Among all identified spacers of CRISPR-Cas system only seven spacers match sequences of phages and plasmids. Furthermore, a conjugative plasmid p7ME01, which belongs to a new IncP-1 ancestral archetype without any accessory mobile elements was found in 78-ME. Our results provide the context to the co-occurrence of diverse defence mechanisms in the genome of Cycloclasticus sp. 78-ME, which protect the genome of this highly specialized PAH-degrader. This study contributes to the further understanding of complex networks established in petroleum-based microbial communities. |
| Source | Environmental microbiology reports 8 (4), pp. 508–519 |
| Keywords | Cycloclasticus sp. 78-MEmultiple defence systems |
| Journal | Environmental microbiology reports |
| Editor | Wiley-Blackwell, [Oxford], Stati Uniti d'America |
| Year | 2016 |
| Type | Articolo in rivista |
| DOI | 10.1111/1758-2229.12424 |
| Authors | Yakimov, Michail M.; Crisafi, Francesca; Messina, Enzo; Smedile, Francesco; Lopatina, Anna; Denaro, Renata; Pieper, Dietmar H.; Golyshin, Peter N.; Giuliano, Laura |
| Text | 371435 2016 10.1111/1758 2229.12424 ISI Web of Science WOS 000379938900011 Cycloclasticus sp. 78 ME multiple defence systems Analysis of defence systems and a conjugative IncP 1 plasmid in the marine polyaromatic hydrocarbons degrading bacterium Cycloclasticus sp 78 ME Yakimov, Michail M.; Crisafi, Francesca; Messina, Enzo; Smedile, Francesco; Lopatina, Anna; Denaro, Renata; Pieper, Dietmar H.; Golyshin, Peter N.; Giuliano, Laura CNR; Russian Acad Sci; HZI Helmholtz Ctr Infect Res; Bangor Univ Marine prokaryotes have evolved a broad repertoire of defence systems to protect their genomes from lateral gene transfer including innate or acquired immune systems and infection induced programmed cell suicide and dormancy. Here we report on the analysis of multiple defence systems present in the genome of the strain Cycloclasticus sp. 78 ME isolated from petroleum deposits of the tanker Amoco Milford Haven . Cycloclasticus are ubiquitous bacteria globally important in polyaromatic hydrocarbons degradation in marine environments. Two defence islands were identified in 78 ME genome the first harbouring CRISPR Cas with toxin antitoxin system, while the second was composed by an array of genes for toxin antitoxin and restriction modification proteins. Among all identified spacers of CRISPR Cas system only seven spacers match sequences of phages and plasmids. Furthermore, a conjugative plasmid p7ME01, which belongs to a new IncP 1 ancestral archetype without any accessory mobile elements was found in 78 ME. Our results provide the context to the co occurrence of diverse defence mechanisms in the genome of Cycloclasticus sp. 78 ME, which protect the genome of this highly specialized PAH degrader. This study contributes to the further understanding of complex networks established in petroleum based microbial communities. 8 Published version Articolo in rivista Wiley Blackwell 1758 2229 Environmental microbiology reports Environmental microbiology reports Environ. microbiol. rep. Environmental microbiology reports enzo.messina MESSINA ENZO mikhail.iakimov IAKIMOV MIKHAIL renata.denaro DENARO RENATA francesco.smedile SMEDILE FRANCESCO francesca.crisafi CRISAFI FRANCESCA |