Scheda di dettaglio – i prodotti della ricerca

DatoValore
TitleIsolation and degradation potential of a cold-adapted oil/PAH-degrading marine bacterial consortium from Kongsfjorden (Arctic region)
AbstractThe temperature is one of the most important factors determining the success of bioremediation processes in marine environment. This study focused on the isolation and characterization of marine hydrocarbons-degrading bacteria from a cold region. Seawater samples were collected in the Ny-lesund Harbor and in the proximity of Kronebreen glacier (Kongsfjorden, Svalbard), and immediately enriched with nutrients and hydrocarbons as sole source of carbon. Samples were maintained at 4 A degrees C for 90 days. Most probable number analyses showed a high number of hydrocarbons degraders from the samples collected in the harbor, while we were not able to detect hydrocarbon degraders in samples collected in the sea-ice region. The isolation was carried out at 4 and 15 A degrees C, and a list of 38 strains was obtained in pure culture using both oil and polycyclic aromatic hydrocarbons as substrates. The selection inferred by the two different substrates was evident as oil favorites the growth of strains belonging to the gamma-proteobacteria: Pseudoalteromonas, Marinobacter, Oleispira, and Alcanivorax genera while naphthalene and phenanthrene selected mostly alpha-proteobacteria: Sphingopyxis, Rhodobacter, and Hyphomonas genera. The isolated have been further selected for the formulation of a microbial consortium to test their potential to degrade both oil and polycyclic aromatic hydrocarbons at low temperature. Results demonstrated that the consortium was very active at 15 A degrees C at which the almost 90 % of TPH were degraded; at 4 A degrees C we were able to detect almost 80 % of degradation. Moreover, parallel single culture of each strain was not as efficient as in consortium.
SourceRendiconti lincei. Scienze fisiche e naturali 27, pp. 261–270
KeywordsArcticBioremediationBacterial isolationHydrocarbons
JournalRendiconti lincei. Scienze fisiche e naturali
EditorSpringer Verlag Italia, Milano, Italia
Year2016
TypeArticolo in rivista
DOI10.1007/s12210-016-0550-6
AuthorsCrisafi, Francesca; Giuliano, Laura; Yakimov, Michail M.; Azzaro, Maurizio; Denaro, Renata
Text371434 2016 10.1007/s12210 016 0550 6 ISI Web of Science WOS 000382669200027 Arctic Bioremediation Bacterial isolation Hydrocarbons Isolation and degradation potential of a cold adapted oil/PAH degrading marine bacterial consortium from Kongsfjorden Arctic region Crisafi, Francesca; Giuliano, Laura; Yakimov, Michail M.; Azzaro, Maurizio; Denaro, Renata Inst Coastal Marine Environm IAMC CNR The temperature is one of the most important factors determining the success of bioremediation processes in marine environment. This study focused on the isolation and characterization of marine hydrocarbons degrading bacteria from a cold region. Seawater samples were collected in the Ny lesund Harbor and in the proximity of Kronebreen glacier Kongsfjorden, Svalbard , and immediately enriched with nutrients and hydrocarbons as sole source of carbon. Samples were maintained at 4 A degrees C for 90 days. Most probable number analyses showed a high number of hydrocarbons degraders from the samples collected in the harbor, while we were not able to detect hydrocarbon degraders in samples collected in the sea ice region. The isolation was carried out at 4 and 15 A degrees C, and a list of 38 strains was obtained in pure culture using both oil and polycyclic aromatic hydrocarbons as substrates. The selection inferred by the two different substrates was evident as oil favorites the growth of strains belonging to the gamma proteobacteria Pseudoalteromonas, Marinobacter, Oleispira, and Alcanivorax genera while naphthalene and phenanthrene selected mostly alpha proteobacteria Sphingopyxis, Rhodobacter, and Hyphomonas genera. The isolated have been further selected for the formulation of a microbial consortium to test their potential to degrade both oil and polycyclic aromatic hydrocarbons at low temperature. Results demonstrated that the consortium was very active at 15 A degrees C at which the almost 90 % of TPH were degraded; at 4 A degrees C we were able to detect almost 80 % of degradation. Moreover, parallel single culture of each strain was not as efficient as in consortium. 27 Published version Articolo in rivista Springer Verlag Italia 2037 4631 Rendiconti lincei. Scienze fisiche e naturali Rendiconti lincei. Scienze fisiche e naturali Rend. lincei, Sci. fis. nat. Rendiconti lincei. Scienze fisiche e naturali. Rendiconti lincei mikhail.iakimov IAKIMOV MIKHAIL renata.denaro DENARO RENATA francesca.crisafi CRISAFI FRANCESCA