| Title | Hepatic gene expression profiles of a non-model cyprinid (Barbus plebejus) chronically exposed to river sediments |
| Abstract | In this study, we characterized the gene expression responses of the Padanian barbel (Barbus plebejus), a native benthivorous cyprinid with a very compromised presence within the fish community of the River Po. Barbel juveniles were exposed in the laboratory to two river sediments reflecting an upstream/downstream gradient of increasing contamination and collected from one of the most anthropized tributaries of the River Po. After 7 months of exposure, hepatic transcriptional changes that were diagnostic of sediment exposure were assessed. We investigated a set of 24 genes involved in xenobiotic biotransformation (cyp1a, gst?, ugt), antioxidant defense (gpx, sod, cat, hsp70), trace metal exposure (mt-I, mt-II), DNA repair (xpa, xpc), apoptosis (bax, casp3), growth (igf2), and steroid (er?, er?1, er?2, ar, vtg) and thyroid (dio1, dio2, tr?, tr?, nis) hormone signaling pathways. In a consistent overall picture, the results showed that long-term sediment exposure mainly increased the levels of mRNAs encoding proteins involved in xenobiotic metabolism, oxidative stress defense, repair of DNA damage and activation of the apoptotic process. Transcript up-regulation of three receptor genes (er?2, ar, tr?), likely representing compensatory responses to antagonistic/toxic effects, was also observed, confirming the exposure to disruptors of the reproductive and thyroidal axes. In contrast to expectations, a few genes showed no response (e.g., casp3) or even downregulation (vtg), further suggesting that the timing of exposure/assessment, potential compensatory effects or post-transcriptional modifications interact to modify the gene expression profiles, particularly during exposure to mixtures of contaminants. |
| Source | Comparative biochemistry and physiology. Toxicology & pharmacology (Print) 196, pp. 27–35 |
| Keywords | gene expressionlong-term exposureendocrine disruptionriver sedimentstoxic effects |
| Journal | Comparative biochemistry and physiology. Toxicology & pharmacology (Print) |
| Editor | Elsevier Science, New York, NY, Stati Uniti d'America |
| Year | 2017 |
| Type | Articolo in rivista |
| DOI | 10.1016/j.cbpc.2017.03.006 |
| Authors | Casatta N.; Stefani F.; Viganò L. |
| Text | 368482 2017 10.1016/j.cbpc.2017.03.006 Scopus 2 s2.0 85014993919 ISI Web of Science WOS 000401376800004 gene expression long term exposure endocrine disruption river sediments toxic effects Hepatic gene expression profiles of a non model cyprinid Barbus plebejus chronically exposed to river sediments Casatta N.; Stefani F.; Vigano L. Water Research Institute, National Research Council of Italy, Via del Mulino 19, Brugherio, MB, 20861, , Italy In this study, we characterized the gene expression responses of the Padanian barbel Barbus plebejus , a native benthivorous cyprinid with a very compromised presence within the fish community of the River Po. Barbel juveniles were exposed in the laboratory to two river sediments reflecting an upstream/downstream gradient of increasing contamination and collected from one of the most anthropized tributaries of the River Po. After 7 months of exposure, hepatic transcriptional changes that were diagnostic of sediment exposure were assessed. We investigated a set of 24 genes involved in xenobiotic biotransformation cyp1a, gst , ugt , antioxidant defense gpx, sod, cat, hsp70 , trace metal exposure mt I, mt II , DNA repair xpa, xpc , apoptosis bax, casp3 , growth igf2 , and steroid er , er 1, er 2, ar, vtg and thyroid dio1, dio2, tr , tr , nis hormone signaling pathways. In a consistent overall picture, the results showed that long term sediment exposure mainly increased the levels of mRNAs encoding proteins involved in xenobiotic metabolism, oxidative stress defense, repair of DNA damage and activation of the apoptotic process. Transcript up regulation of three receptor genes er 2, ar, tr , likely representing compensatory responses to antagonistic/toxic effects, was also observed, confirming the exposure to disruptors of the reproductive and thyroidal axes. In contrast to expectations, a few genes showed no response e.g., casp3 or even downregulation vtg , further suggesting that the timing of exposure/assessment, potential compensatory effects or post transcriptional modifications interact to modify the gene expression profiles, particularly during exposure to mixtures of contaminants. 196 Published version http //www.scopus.com/record/display.url eid=2 s2.0 85014993919 origin=inward Hepatic gene expression profiles of a non model cyprinid Barbus plebejus chronically exposed to river sediments file articolo in formato pdf Barbus gene express CBP part C 2017.pdf Articolo in rivista Elsevier Science 1532 0456 Comparative biochemistry and physiology. Toxicology pharmacology Print Comparative biochemistry and physiology. Toxicology pharmacology Print Comp. biochem. physiol., Toxicol. pharmacol. Print Comparative biochemistry and physiology. Print Toxicology pharmacology Print Toxicology and pharmacology Print CBP. Print Toxicology pharmacology Print Comparative biochemistry and physiology. Print C, Print Toxicology pharmacology Print Comparative biochemistry and physiology. Print Part C Print CASATTA NADIA fabrizio.stefani STEFANI FABRIZIO luigi.vigano VIGANO LUIGI |
