| Title | Antibiotic resistance genes fate and removal by a technological treatment solution for water reuse in agriculture |
| Abstract | In order to mitigate the potential effects on the human health which are associated to the use of treated wastewater in agriculture, antibiotic resistance genes (ARGs) are required to be carefully monitored in wastewater reuse processes and their spread should be prevented by the development of efficient treatment technologies. Objective of this study was the assessment of ARGs reduction efficiencies of a novel technological treatment solution for agricultural reuse of municipal wastewaters. The proposed solution comprises an advanced biological treatment (Sequencing Batch Biofilter Granular Reactor, SBBGR), analysed both al laboratory and pilot scale,
followed by sand filtration and two different disinfection final stages: ultraviolet light (UV) radiation and peracetic acid (PAA) treatments. By Polymerase Chain Reaction (PCR), the presence of 9 ARGs (ampC, mecA, ermB, sul1, sul2, tetA, tetO, tetW, vanA) were analysed and by quantitative PCR (qPCR) their removal was determined. The obtained results were compared to the reduction of total bacteria (16S rDNA gene) and of a faecal contamination indicator (Escherichia coli uidA gene). Only four of the analysed genes (ermB, sul1, sul2, tetA) were detected in raw wastewater and their abundance was estimated to be 3.4±0.7 x104 - 9.6±0.5 x109 and 1.0±0.3 x103 to 3.0±0.1 x107 gene copies/mL in raw and treated wastewaters, respectively. The results show that SBBGR technology is promising for the reduction of ARGs, achieving stable removal performance ranging from1.0±0.4 to 2.8±0.7 log units,which is comparable to or higher than that reported for conventional activated
sludge treatments. No reduction of the ARGs amount normalized to the total bacteria content (16S rDNA),was instead obtained, indicating that these genes are removed together with total bacteria and not specifically eliminated. Enhanced ARGs removalwas obtained by sand filtration,while no reductionwas achieved by both UV and PAA disinfection treatments tested in our study. |
| Source | Science of the total environment 571, pp. 809–818 |
| Keywords | Antibiotic resistance genesSequencing Batch Biofilter Granular ReactorWater reuseDisinfectionWastewater treatmentE.coli |
| Journal | Science of the total environment |
| Editor | Elsevier, Lausanne ;, Paesi Bassi |
| Year | 2016 |
| Type | Articolo in rivista |
| DOI | 10.1016/j.scitotenv.2016.07.055 |
| Authors | Luprano M.L.; De Sanctis M.; Del Moro G.; Di Iaconi C.; Lopez A.; Levantesi C. |
| Text | 367374 2016 10.1016/j.scitotenv.2016.07.055 Scopus 2 s2.0 84978897588 ISI Web of Science WOS WOS 000383930400081 Antibiotic resistance genes Sequencing Batch Biofilter Granular Reactor Water reuse Disinfection Wastewater treatment E.coli Antibiotic resistance genes fate and removal by a technological treatment solution for water reuse in agriculture Luprano M.L.; De Sanctis M.; Del Moro G.; Di Iaconi C.; Lopez A.; Levantesi C. Istituto di Ricerca Sulle Acque del CNR CNR IRSA , Via Salaria Km 29.600, Monterotondo, RM, 00015, Italia; Istituto di Ricerca Sulle Acque del CNR CNR IRSA , Via F. De Blasio 5, Bari, 70123, Italia In order to mitigate the potential effects on the human health which are associated to the use of treated wastewater in agriculture, antibiotic resistance genes ARGs are required to be carefully monitored in wastewater reuse processes and their spread should be prevented by the development of efficient treatment technologies. Objective of this study was the assessment of ARGs reduction efficiencies of a novel technological treatment solution for agricultural reuse of municipal wastewaters. The proposed solution comprises an advanced biological treatment Sequencing Batch Biofilter Granular Reactor, SBBGR , analysed both al laboratory and pilot scale, followed by sand filtration and two different disinfection final stages ultraviolet light UV radiation and peracetic acid PAA treatments. By Polymerase Chain Reaction PCR , the presence of 9 ARGs ampC, mecA, ermB, sul1, sul2, tetA, tetO, tetW, vanA were analysed and by quantitative PCR qPCR their removal was determined. The obtained results were compared to the reduction of total bacteria 16S rDNA gene and of a faecal contamination indicator Escherichia coli uidA gene . Only four of the analysed genes ermB, sul1, sul2, tetA were detected in raw wastewater and their abundance was estimated to be 3.4±0.7 x104 9.6±0.5 x109 and 1.0±0.3 x103 to 3.0±0.1 x107 gene copies/mL in raw and treated wastewaters, respectively. The results show that SBBGR technology is promising for the reduction of ARGs, achieving stable removal performance ranging from1.0±0.4 to 2.8±0.7 log units,which is comparable to or higher than that reported for conventional activated sludge treatments. No reduction of the ARGs amount normalized to the total bacteria content 16S rDNA ,was instead obtained, indicating that these genes are removed together with total bacteria and not specifically eliminated. Enhanced ARGs removalwas obtained by sand filtration,while no reductionwas achieved by both UV and PAA disinfection treatments tested in our study. 571 Published version http //www.scopus.com/record/display.url eid=2 s2.0 84978897588 origin=inward Antibiotic resistance genes fate and removal by a technological treatment solution for water reuse in agriculture 2016 luprano et al 1 s2.0 S0048969716315005 main.pdf Articolo in rivista Elsevier 0048 9697 Science of the total environment Science of the total environment Sci. total environ. LUPRANO MARIA LAURA claudio.diiaconi DI IACONI CLAUDIO caterina.levantesi LEVANTESI CATERINA marco.desanctis DE SANCTIS MARCO WATER4CROPS Integrating bio treated wastewater with enhanced water use efficiency to support the Green Economy in EU and India |
