| Abstract | Ammonia-oxidizing Archaea (AOA) play an important role in the oxidation of ammonia in terrestrial, marine, and geothermal habitats, as confirmed by a number of studies specifically focused on those environments. Much less is known about the ecological role of AOA in freshwaters. In order to reach a high resolution at the Thaumarchaea community level, the probe MGI-535 was specifically designed for this study and applied to fluorescence in situ hybridization and catalyzed reporter deposition (CARD-FISH) analysis. We then applied it to a fine analysis of diversity and relative abundance of AOA in the deepest layers of the oligotrophic Lake Maggiore, confirming previous published results of AOA presence, but showing differences in abundance and distribution within the water column without significant seasonal trends with respect to Bacteria. Furthermore, phylogenetic analysis of AOA clone libraries from deep lake water and from a lake tributary, River Maggia, suggested the riverine origin of AOA of the deep hypolimnion of the lake. |
| Text | 366825 2015 10.1155/2015/590434 ISI Web of Science WOS 000360757100001 Scopus 2 s2.0 84941248401 Thaumarchaea; Lago Maggiore Ecology and distribution of Thaumarchaea in the Deep Hypolimnion of Lake Maggiore Coci, Manuela; Odermatt, Nina; Salcher, Michaela M.; Pernthaler, Jakob; Corno, Gianluca Coci, Manuela Microb co, Association for Microbial Ecology, Catania, Italy; Odermatt, Nina e Pernthaler, Jakob Limnological Station, Institute of Plant Biology, University of Zurich, Kilchberg, Switzerland; Salcher, Michaela M. Institute of Hydrobiology, Biology Centre CAS, Ceske Budejovice, Czech Republic; Corno, Gianluca ISE VB Ammonia oxidizing Archaea AOA play an important role in the oxidation of ammonia in terrestrial, marine, and geothermal habitats, as confirmed by a number of studies specifically focused on those environments. Much less is known about the ecological role of AOA in freshwaters. In order to reach a high resolution at the Thaumarchaea community level, the probe MGI 535 was specifically designed for this study and applied to fluorescence in situ hybridization and catalyzed reporter deposition CARD FISH analysis. We then applied it to a fine analysis of diversity and relative abundance of AOA in the deepest layers of the oligotrophic Lake Maggiore, confirming previous published results of AOA presence, but showing differences in abundance and distribution within the water column without significant seasonal trends with respect to Bacteria. Furthermore, phylogenetic analysis of AOA clone libraries from deep lake water and from a lake tributary, River Maggia, suggested the riverine origin of AOA of the deep hypolimnion of the lake. 2015 Published version Articolo 2015_A0_053.pdf Articolo in rivista Hindawi Publishing Corporation 1472 3646 Archaea Archaea gianluca.corno CORNO GIANLUCA |
