| Title | Effect of starvation on survival and virulence expression of Aeromonas hydrophila from different sources |
| Abstract | Aeromonas hydrophila is an aquatic bacterium responsible for several human illnesses. The aim of this work was to investigate the survival ability and virulence expression of two strains from different sources (fish, strain 87 and surface water, strain LS) maintained in a seawater microcosm. The strains were analyzed for the total and viable bacterial counts, adhesion ability to Hep-2 cells and aerA gene expression by qPCR throughout the experiment (35 days). Both strains reached a putative VBNC state and lost adhesive properties but exhibited a different behavior in the expression of aerA. This could be due to the different origin of the two strains; the former adapted to a habitat rich of nutrient and the latter already used to survive in a more hostile environment. Moreover, our results indicate that the quantitative determination of aerA mRNA can be a useful indicator of virulence expression under stress conditions. |
| Source | Archives of microbiology 197 (3), pp. 431–438 |
| Keywords | Aeromonas hydrophilaqRT-PCRVirulenceNutritional stressIsolation sourcesVBNC |
| Journal | Archives of microbiology |
| Editor | Springer, Berlin, Germania |
| Year | 2015 |
| Type | Articolo in rivista |
| DOI | 10.1007/s00203-014-1074-z |
| Authors | Casabianca, Anna; Orlandi, Chiara; Barbieri, Federica; Sabatini, Luigia; Di Cesare, Andrea; Sisti, Davide; Pasquaroli, Sonia; Magnani, Mauro; Citterio, Barbara |
| Text | 366813 2015 10.1007/s00203 014 1074 z ISI Web of Science WOS 000351402200008 Scopus 2 s2.0 84925498807 Aeromonas hydrophila qRT PCR Virulence Nutritional stress Isolation sources VBNC Effect of starvation on survival and virulence expression of Aeromonas hydrophila from different sources Casabianca, Anna; Orlandi, Chiara; Barbieri, Federica; Sabatini, Luigia; Di Cesare, Andrea; Sisti, Davide; Pasquaroli, Sonia; Magnani, Mauro; Citterio, Barbara Division of Biochemistry and Molecular Biology, Department of Biomolecular Sciences, University of Urbino Carlo Bo ; Division of Toxicology, Hygienistic and Environmental Sciences, Department of Biomolecular Sciences, University of Urbino Carlo Bo ; CNR ISE, Division of Pharmacology and Pharmacognosy, Department of Biomolecular Sciences, University of Urbino Carlo Bo ; Department of Life and Environmental Sciences, Polytechnic University of Marche, Ancona Aeromonas hydrophila is an aquatic bacterium responsible for several human illnesses. The aim of this work was to investigate the survival ability and virulence expression of two strains from different sources fish, strain 87 and surface water, strain LS maintained in a seawater microcosm. The strains were analyzed for the total and viable bacterial counts, adhesion ability to Hep 2 cells and aerA gene expression by qPCR throughout the experiment 35 days . Both strains reached a putative VBNC state and lost adhesive properties but exhibited a different behavior in the expression of aerA. This could be due to the different origin of the two strains; the former adapted to a habitat rich of nutrient and the latter already used to survive in a more hostile environment. Moreover, our results indicate that the quantitative determination of aerA mRNA can be a useful indicator of virulence expression under stress conditions. 197 Published version Articolo 2015_A0_051.pdf Articolo in rivista Springer 0302 8933 Archives of microbiology Archives of microbiology Arch. microbiol. Archives of microbiology. Archives of microbiology Print andrea.dicesare DI CESARE ANDREA |
