| Title | Polyhydroxyalkanoate as a slow-release carbon source for in situ bioremediation of contaminated aquifers: From laboratory investigation to pilot-scale testing in the field. |
| Abstract | A pilot-scale study aiming to evaluate the potential use of poly-3-hydroxy-butyrate (PHB) as an electron donor source for in situ bioremediation of chlorinated hydrocarbons in groundwater was conducted. Compared with commercially available electron donors, PHB offers a restricted fermentation pathway (i.e., through acetic acid and molecular hydrogen) by avoiding the formation of any residual carbon that could potentially spoil groundwater quality. The pilot study was carried out at an industrial site in Italy, heavily contaminated by different chlorinated aliphatic hydrocarbons (CAHs). Prior to field testing, PHB was experimentally verified as a suitable electron donor for biological reductive dechlorination processes at the investigated site by microcosm studies carried out on site aquifer material and measuring the quantitative transformation of detected CAHs to ethene. Owing to the complex geological characteristics of the aquifer, the use of a groundwater circulation well (GCW) was identified as a potential strategy to enable effective delivery and distribution of electron donors in less permeable layers and to mobilise contaminants. A 3-screened, 30-m-deep GCW coupled with an external treatment unit was installed at the site. The effect of PHB fermentation products on the in situ reductive dechlorination processes were evaluated by quantitative real-time polymerase chain reaction (qPCR). The results from the first 4 months of operation clearly demonstrated that the PHB fermentation products were effectively delivered to the aquifer and positively influenced the biological dechlorination activity. Indeed, an increased abundance of Dehalococcoides mccartyi (up to 6.6 fold) and reduced CAH concentrations at the installed monitoring wells were observed. |
| Source | New biotechnology (Online) Volume 37 (PartA) |
| Keywords | Poly (3-hydroxybutyrate)Real-time PCRGroundwater circulation wellBiological reductive dechlorinationChlorinated aliphatic hydrocarbons |
| Journal | New biotechnology (Online) |
| Editor | Elsevier, Amsterdam, Paesi Bassi |
| Year | 2017 |
| Type | Articolo in rivista |
| DOI | 10.1016/j.nbt.2016.11.004 |
| Authors | Pierro L, Matturro B, Rossetti S, Sagliaschi M, Sucato S, Alesi E, Bartsch E, Arjmand F, Papini MP |
| Text | 362979 2017 10.1016/j.nbt.2016.11.004 ISI Web of Science WOS WOS 000401208000007 Poly 3 hydroxybutyrate Real time PCR Groundwater circulation well Biological reductive dechlorination Chlorinated aliphatic hydrocarbons Polyhydroxyalkanoate as a slow release carbon source for in situ bioremediation of contaminated aquifers From laboratory investigation to pilot scale testing in the field. Pierro L, Matturro B, Rossetti S, Sagliaschi M, Sucato S, Alesi E, Bartsch E, Arjmand F, Papini MP Department of Chemistry Stanislao Cannizzaro , Sapienza University of Rome, Italy. Water Research Institute National Research Council, IRSACNR, Monterotondo, Italy. Water Research Institute National Research Council, IRSACNR, Monterotondo, Italy. EDF Fenice SpA, Torino, Italy. EDF Fenice SpA, Torino, Italy. IEG Technologie GmbH Gruibingen, Germany. Department of Chemistry Stanislao Cannizzaro , Sapienza University of Rome, Italy. A pilot scale study aiming to evaluate the potential use of poly 3 hydroxy butyrate PHB as an electron donor source for in situ bioremediation of chlorinated hydrocarbons in groundwater was conducted. Compared with commercially available electron donors, PHB offers a restricted fermentation pathway i.e., through acetic acid and molecular hydrogen by avoiding the formation of any residual carbon that could potentially spoil groundwater quality. The pilot study was carried out at an industrial site in Italy, heavily contaminated by different chlorinated aliphatic hydrocarbons CAHs . Prior to field testing, PHB was experimentally verified as a suitable electron donor for biological reductive dechlorination processes at the investigated site by microcosm studies carried out on site aquifer material and measuring the quantitative transformation of detected CAHs to ethene. Owing to the complex geological characteristics of the aquifer, the use of a groundwater circulation well GCW was identified as a potential strategy to enable effective delivery and distribution of electron donors in less permeable layers and to mobilise contaminants. A 3 screened, 30 m deep GCW coupled with an external treatment unit was installed at the site. The effect of PHB fermentation products on the in situ reductive dechlorination processes were evaluated by quantitative real time polymerase chain reaction qPCR . The results from the first 4 months of operation clearly demonstrated that the PHB fermentation products were effectively delivered to the aquifer and positively influenced the biological dechlorination activity. Indeed, an increased abundance of Dehalococcoides mccartyi up to 6.6 fold and reduced CAH concentrations at the installed monitoring wells were observed. Volume 37 Published version https //doi.org/10.1016/j.nbt.2016.11.004 Pierro et al., 2017 Pierro et al.,2017.pdf Articolo in rivista Elsevier 1876 4347 New biotechnology Online New biotechnology Online New biotechnology. Online simona.rossetti ROSSETTI SIMONA bruna.matturro MATTURRO BRUNA |
