| Title | Degradation of a fluoroquinolone antibiotic in an urbanized stretch of the river Tiber |
| Abstract | The widespread detection of antibiotics in terrestrial and aquatic systems has engendered significant scientific and regulatory concern. Overall, knowledge concerning the ecotoxicology and sub-lethal effects in water is scarce, but some experimental studies show that antibiotics can induce pathogen resistance and they can also have detrimental effects on natural microbial communities and their key functions.
The main aim of this study was to investigate the occurrence of the biodegradation and photodegradation processes of the fluoroquinolone ciprofloxacin (CIP) in the River Tiber waters, in a stretch highly impacted from human pressure. Two set of microcosms consisting of river water containing the natural microbial community and treated with 500 ?g/L of CIP in absence or presence of UV-light were performed. Moreover, some microcosms were filled with river water previously sterilized and then treated with the antibiotic. The combined experimental set made it possible to evaluate if the antibiotic CIP could be photodegraded and/or biodegraded.
CIP residual concentrations were measured over time by using HPLC coupled to fluorescence detection (FLD) and the effects of the antibiotic on the natural microbial community were assessed in terms of live cell abundance. The key role of light in CIP disappearance was confirmed, but also its biodegradation in natural river water was demonstrated. In fact, differently from other experiments we found a higher degradation rate (DT50 = 10.4 d), in presence of both light and the natural river bacterial populations than in the same sterilized river water (DT50 = 18.4 d). Moreover, even in the dark, a partial CIP biodegradation was also observed (DT50 = 177 d). The overall results were supported by the increase in live cell numbers with the decrease of CIP concentrations both in the dark and light condition. |
| Source | Microchemical journal (Print) 136, pp. 43–48 |
| Keywords | ciprofloxacinriver ecosystembiodegradationphotodegradationpersitencelive cell abundance |
| Journal | Microchemical journal (Print) |
| Editor | Academic Press [etc.], New York,, Stati Uniti d'America |
| Year | 2018 |
| Type | Articolo in rivista |
| DOI | 10.1016/j.microc.2016.12.008 |
| Authors | Barra Caracciolo A, Grenni P, Rauseo J, Ademollo N, Cardoni M, Rolando L, Patrolecco L |
| Text | 362524 2018 10.1016/j.microc.2016.12.008 ciprofloxacin river ecosystem biodegradation photodegradation persitence live cell abundance Degradation of a fluoroquinolone antibiotic in an urbanized stretch of the river Tiber Barra Caracciolo A, Grenni P, Rauseo J, Ademollo N, Cardoni M, Rolando L, Patrolecco L Water Research Institute, National Research Council, Via Salaria km 29.300, 00015 Monterotondo Scalo RM , Italy The widespread detection of antibiotics in terrestrial and aquatic systems has engendered significant scientific and regulatory concern. Overall, knowledge concerning the ecotoxicology and sub lethal effects in water is scarce, but some experimental studies show that antibiotics can induce pathogen resistance and they can also have detrimental effects on natural microbial communities and their key functions. The main aim of this study was to investigate the occurrence of the biodegradation and photodegradation processes of the fluoroquinolone ciprofloxacin CIP in the River Tiber waters, in a stretch highly impacted from human pressure. Two set of microcosms consisting of river water containing the natural microbial community and treated with 500 g/L of CIP in absence or presence of UV light were performed. Moreover, some microcosms were filled with river water previously sterilized and then treated with the antibiotic. The combined experimental set made it possible to evaluate if the antibiotic CIP could be photodegraded and/or biodegraded. CIP residual concentrations were measured over time by using HPLC coupled to fluorescence detection FLD and the effects of the antibiotic on the natural microbial community were assessed in terms of live cell abundance. The key role of light in CIP disappearance was confirmed, but also its biodegradation in natural river water was demonstrated. In fact, differently from other experiments we found a higher degradation rate DT50 = 10.4 d , in presence of both light and the natural river bacterial populations than in the same sterilized river water DT50 = 18.4 d . Moreover, even in the dark, a partial CIP biodegradation was also observed DT50 = 177 d . The overall results were supported by the increase in live cell numbers with the decrease of CIP concentrations both in the dark and light condition. 136 Published version 13/12/2016 Degradation of a fluoroquinolone antibiotic antibiotic in an urbanized stretch of the river Tiber Paper Microchem2018Ciprofloxacin.pdf Articolo in rivista Academic Press etc. 0026 265X Microchemical journal Print Microchemical journal Print Microchemical journal. Print jasmin.rauseo RAUSEO JASMIN CARDONI MARTINA luisa.patrolecco PATROLECCO LUISA anna.barracaracciolo BARRA CARACCIOLO ANNA paola.grenni GRENNI PAOLA nicoletta.ademollo ADEMOLLO NICOLETTA MICROCOKIT Microbial Community based sequencing analysis linked to anthropogenic pressures MicroCoKit to address the water quality TA.P04.005.011 Vulnerabilita degli ecosistemi delle acque sotterranee e attenuazione naturale degli inquinanti nel suolo e nel sottosuolo |
