Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | Algal ecotoxicological testing of groundwater priority contaminants using closed-system technique |
| Abstract | While toxicity testing should be an integral part of the groundwater bioremediation projects, only few studies have attempted to include ecotoxicological tests, since standard testing procedures have not been developed to suit some of the groundwater priority contaminants. In fact, volatile organic compounds (VOCs), such as chlorinated aliphatic hydrocarbons (CAHs), belong to the most frequently found contaminants in European groundwaters. The development of a sensitive ecotoxicological procedure being appropriate for VOCs while relatively easy to be performed is therefore regarded as a current research need. With this aim, an algal test procedure has been set-up within a bioremediation project aimed at the evaluation of the efficiency of a bioelectrochemical technique applied to groundwaters historically contaminated by CAHs. The procedure was developed using a closed-system, as required by testing VOCs. Since a major point when using exposure systems without gas exchange is linked to the carbon dioxide limitation, we selected to supply CO2 as bicarbonate enrichment. Closed-flasks completely filled with bicarbonate-added test solutions were stoppered with parafilm lids, and connected to a magnetic stirrer mechanism to guarantee the homogeneous suspension of the growing algal cells during the 72 hour-exposure. The increased efficiency of the closed versus the standard procedure was evaluated by the simultaneous use of both methods to test standard chemicals as well as contaminated groundwater samples. Finally, the ability of the closed-system to detect the detoxification effect of the groundwater dechlorination treatment was evaluated by testing pre and post-treatment samples from a laboratory-scale bioelectrochemical reactor. Based on the obtained results, revealing the important advantage of the closed versus the standard system, the proposed ecotoxicological technique proved appropriate for testing groundwater priority contaminants. The combination of its characteristics of simplicity and sensitivity makes it suitable to be routinely used for groundwater monitoring, successfully improving bioremediation studies. Particularly, based on its characteristics, such a sensitive and simple test design could be suitable for checking innovative groundwater bioremediation technologies. |
| Source | 18th International Symposium on Environmental Pollution and its Impact on Life in the Mediterranean Region, Crete (GR), 26 - 30/09/2015 |
| Keywords | Algal toxicity testingclosed test systemCAHsgroundwater bioremediation |
| Year | 2015 |
| Type | Contributo in atti di convegno |
| Authors | Mingazzini M., Palumbo M.T. |
| Text | 361614 2015 Algal toxicity testing closed test system CAHs groundwater bioremediation Algal ecotoxicological testing of groundwater priority contaminants using closed system technique Mingazzini M., Palumbo M.T. IRSA CNR While toxicity testing should be an integral part of the groundwater bioremediation projects, only few studies have attempted to include ecotoxicological tests, since standard testing procedures have not been developed to suit some of the groundwater priority contaminants. In fact, volatile organic compounds VOCs , such as chlorinated aliphatic hydrocarbons CAHs , belong to the most frequently found contaminants in European groundwaters. The development of a sensitive ecotoxicological procedure being appropriate for VOCs while relatively easy to be performed is therefore regarded as a current research need. With this aim, an algal test procedure has been set up within a bioremediation project aimed at the evaluation of the efficiency of a bioelectrochemical technique applied to groundwaters historically contaminated by CAHs. The procedure was developed using a closed system, as required by testing VOCs. Since a major point when using exposure systems without gas exchange is linked to the carbon dioxide limitation, we selected to supply CO2 as bicarbonate enrichment. Closed flasks completely filled with bicarbonate added test solutions were stoppered with parafilm lids, and connected to a magnetic stirrer mechanism to guarantee the homogeneous suspension of the growing algal cells during the 72 hour exposure. The increased efficiency of the closed versus the standard procedure was evaluated by the simultaneous use of both methods to test standard chemicals as well as contaminated groundwater samples. Finally, the ability of the closed system to detect the detoxification effect of the groundwater dechlorination treatment was evaluated by testing pre and post treatment samples from a laboratory scale bioelectrochemical reactor. Based on the obtained results, revealing the important advantage of the closed versus the standard system, the proposed ecotoxicological technique proved appropriate for testing groundwater priority contaminants. The combination of its characteristics of simplicity and sensitivity makes it suitable to be routinely used for groundwater monitoring, successfully improving bioremediation studies. Particularly, based on its characteristics, such a sensitive and simple test design could be suitable for checking innovative groundwater bioremediation technologies. Published version Mingazzini M., Palumbo M.T., 2015. Algal ecotoxicological testing of groundwater priority contaminants using closed system technique. 18th MESAEP Symposium, Crete GR , September 2015, Book of abstracts, 253 18th International Symposium on Environmental Pollution and its Impact on Life in the Mediterranean Region Crete GR 26 30/09/2015 Internazionale Contributo Algal ecotoxicological testing of groundwater priority contaminants using closed system technique MingazziniPalumbo2015_MESAEP Tox.pdf Contributo in atti di convegno mariateresa.palumbo PALUMBO MARIA TERESA marina.mingazzini MINGAZZINI MARINA |