Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | Ultra-trace levels analysis of microcystins and nodularin in surface water by on-line solid-phase extraction with high-performance liquid chromatography tandem mass spectrometry |
| Abstract | An on-line solid phase extraction coupled with high-performance liquid chromatography in tandem with mass spectrometry (on-line SPE/HPLC/MS-MS) method for the determination of five microcystins and nodularin in surface waters at submicrogram per liter concentrations has been optimized. Maximum recoveries were achieved by carefully optimizing the extraction sample volume, loading solvent, wash solvent, and pH of the sample. The developed method was also validated according to both UNI EN ISO IEC 17025 and UNICHIM guidelines. Specifically, ten analytical runs were performed at three different concentration levels using a reference mix solution containing the six analytes. The method was applied for monitoring the concentrations of microcystins and nodularin in real surface water during a sampling campaign of 9 months in which the ELISA method was used as standard official method. The results of the two methods were compared showing good agreement when the highest concentration values of MCs were found. [Figure not available: see fulltext.] |
| Source | Analytical & bioanalytical chemistry (Print) 408, pp. 4063–4071 |
| Keywords | Microcystins . Demethylated microcystins . On-line SPE/HPLC/MS-MS |
| Journal | Analytical & bioanalytical chemistry (Print) |
| Editor | Springer, Heidelberg, Germania |
| Year | 2016 |
| Type | Articolo in rivista |
| DOI | 10.1007/s00216-016-9495-y |
| Authors | Balest L.; Murgolo S.; Sciancalepore L.; Montemurro P.; Abis P.P.; Pastore C.; Mascolo G. |
| Text | 359824 2016 10.1007/s00216 016 9495 y Scopus 2 s2.0 84962725397 Microcystins . Demethylated microcystins . On line SPE/HPLC/MS MS Ultra trace levels analysis of microcystins and nodularin in surface water by on line solid phase extraction with high performance liquid chromatography tandem mass spectrometry Balest L.; Murgolo S.; Sciancalepore L.; Montemurro P.; Abis P.P.; Pastore C.; Mascolo G. Acquedotto Pugliese S.p.A., Area Controllo Qualita, Direzione Operativa DOPRI , Viale Emanuele Orlando, Bari, 70121, Italy; Consiglio Nazionale delle Ricerche, Istituto di Ricerca Sulle Acque, Viale F. De Blasio 5, Bari, 70132, Italy An on line solid phase extraction coupled with high performance liquid chromatography in tandem with mass spectrometry on line SPE/HPLC/MS MS method for the determination of five microcystins and nodularin in surface waters at submicrogram per liter concentrations has been optimized. Maximum recoveries were achieved by carefully optimizing the extraction sample volume, loading solvent, wash solvent, and pH of the sample. The developed method was also validated according to both UNI EN ISO IEC 17025 and UNICHIM guidelines. Specifically, ten analytical runs were performed at three different concentration levels using a reference mix solution containing the six analytes. The method was applied for monitoring the concentrations of microcystins and nodularin in real surface water during a sampling campaign of 9 months in which the ELISA method was used as standard official method. The results of the two methods were compared showing good agreement when the highest concentration values of MCs were found. Figure not available see fulltext. 408 Published version http //www.scopus.com/record/display.url eid=2 s2.0 84962725397 origin=inward Articolo in rivista Springer 1618 2642 Analytical bioanalytical chemistry Print Analytical bioanalytical chemistry Print Anal. bioanal. chem. Print Analytical bioanalytical chemistry. Print Analytical and bioanalytical chemistry Print sapia.murgolo MURGOLO SAPIA carlo.pastore PASTORE CARLO giuseppe.mascolo MASCOLO GIUSEPPE |