Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | First evidence on the occurrence and dynamics of Dehalococcoides mccartyi PCB-dechlorinase genes in marine sediment during Aroclor1254 reductive dechlorination |
| Abstract | The present study evaluates the PCB-dehalorespiring capabilities and dynamics of indigenous Dehalococcoides mccartyi population in a PCB contaminated marine sediment. Specialized PCB-dechlorinase genes pcbA1, pcbA4 and pcbA5 previously characterized in pure cultures of D. mccartyi, were here found for the first time in environmental samples. Reductive dechlorination was stimulated by spiking Aroclor1254 to the sediment and by imposing strictly anaerobic conditions both with and without bioaugmentation with a Dehalococcoides mccartyi enrichment culture. In line with the contaminant dechlorination kinetics, Dehalococcoides population increased during the entire incubation period showing growth yields of 4.94E + 07 Dehalococcoides per ?mol Cl- 1 and 7.30E + 05 Dehalococcoides per ?mol Cl- 1 in the marine sediment with and without bioaugmentation respectively. The pcbA4 and pcbA5 dechlorinase genes, and to a lesser extent pcbA1 gene, were enriched during the anaerobic incubation suggesting their role in Aroclor1254 dechlorination under salinity conditions. |
| Source | Marine pollution bulletin. |
| Keywords | Polychlorinated biphenyls; Reductive dechlorination; PCB-dechlorinase genes; Dehalococcoides mccartyi; Aroclor1254; Marine sediment |
| Journal | Marine pollution bulletin. |
| Editor | Macmillan,, London, Regno Unito |
| Year | 2016 |
| Type | Articolo in rivista |
| DOI | 10.1016/j.marpolbul.2016.08.021 |
| Authors | Matturro Bruna, Di Lenola Martina, Ubaldi Carla, Rossetti Simona |
| Text | 359314 2016 10.1016/j.marpolbul.2016.08.021 Polychlorinated biphenyls; Reductive dechlorination; PCB dechlorinase genes; Dehalococcoides mccartyi; Aroclor1254; Marine sediment First evidence on the occurrence and dynamics of Dehalococcoides mccartyi PCB dechlorinase genes in marine sediment during Aroclor1254 reductive dechlorination Matturro Bruna, Di Lenola Martina, Ubaldi Carla, Rossetti Simona Water Research Institute, IRSA CNR, Via Salaria km 29,300, Monterotondo RM , Italy ENEA, Technical Unit for Environmental Characterization, Prevention and Remediation, UTPRA, C. R. Casaccia, Italy The present study evaluates the PCB dehalorespiring capabilities and dynamics of indigenous Dehalococcoides mccartyi population in a PCB contaminated marine sediment. Specialized PCB dechlorinase genes pcbA1, pcbA4 and pcbA5 previously characterized in pure cultures of D. mccartyi, were here found for the first time in environmental samples. Reductive dechlorination was stimulated by spiking Aroclor1254 to the sediment and by imposing strictly anaerobic conditions both with and without bioaugmentation with a Dehalococcoides mccartyi enrichment culture. In line with the contaminant dechlorination kinetics, Dehalococcoides population increased during the entire incubation period showing growth yields of 4.94E 07 Dehalococcoides per mol Cl 1 and 7.30E 05 Dehalococcoides per mol Cl 1 in the marine sediment with and without bioaugmentation respectively. The pcbA4 and pcbA5 dechlorinase genes, and to a lesser extent pcbA1 gene, were enriched during the anaerobic incubation suggesting their role in Aroclor1254 dechlorination under salinity conditions. Published version http //www.sciencedirect.com/science/article/pii/S0025326X16306531 Articolo in rivista Macmillan, 0025 326X Marine pollution bulletin. Marine pollution bulletin. Mar. Pollut. Bull. Marine pollution bulletin. simona.rossetti ROSSETTI SIMONA bruna.matturro MATTURRO BRUNA |