Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | In situ detection of alkB2 gene involved in Alcanivorax borkumensis SK2(T) hydrocarbon biodegradation |
| Abstract | This study aimed to develop a new assay based on the whole cell hybridization in order to monitor alkane hydroxylase genes (alkB system) of the marine bacterium Alcanivorax borkumensis SK2T commonly reported as the predominant microorganism responsible for the biodegradation of n-alkanes which are the major fraction of petroleum hydrocarbons. The assay based on the whole cell hybridization targeting alkB2 gene was successfully developed and calibrated on a pure culture of Alcanivorax borkumensis SK2T with a detection efficiency up to 80%. The approach was further successfully validated on hydrocarbon-contaminated seawater and provided cells abundance (6.74E + 04 alkB2-carrying cells mL- 1) higher of about one order of magnitude than those obtained by qPCR (4.96E + 03 alkB2 gene copies mL- 1). This study highlights the validity of the assay for the detection at single cell level of key-functional genes involved in the biodegradation of n-alkanes. |
| Source | Marine pollution bulletin. 110 (1), pp. 378–382 |
| Keywords | Alcanivorax borkumensis SK2(T)Petroleum-polluted seawatern-AlkanesGeneCARD-FISHalkB2 gene |
| Journal | Marine pollution bulletin. |
| Editor | Macmillan,, London, Regno Unito |
| Year | 2016 |
| Type | Articolo in rivista |
| DOI | 10.1016/j.marpolbul.2016.06.038 |
| Authors | Matturro, Bruna; Frascadore, Emanuela; Cappello, Simone; Genovese, Mariella; Rossetti, Simona |
| Text | 359313 2016 10.1016/j.marpolbul.2016.06.038 ISI Web of Science WOS 000382339900052 Alcanivorax borkumensis SK2 T Petroleum polluted seawater n Alkanes GeneCARD FISH alkB2 gene In situ detection of alkB2 gene involved in Alcanivorax borkumensis SK2 T hydrocarbon biodegradation Matturro, Bruna; Frascadore, Emanuela; Cappello, Simone; Genovese, Mariella; Rossetti, Simona IRSA CNR; IAMC CNR This study aimed to develop a new assay based on the whole cell hybridization in order to monitor alkane hydroxylase genes alkB system of the marine bacterium Alcanivorax borkumensis SK2T commonly reported as the predominant microorganism responsible for the biodegradation of n alkanes which are the major fraction of petroleum hydrocarbons. The assay based on the whole cell hybridization targeting alkB2 gene was successfully developed and calibrated on a pure culture of Alcanivorax borkumensis SK2T with a detection efficiency up to 80%. The approach was further successfully validated on hydrocarbon contaminated seawater and provided cells abundance 6.74E 04 alkB2 carrying cells mL 1 higher of about one order of magnitude than those obtained by qPCR 4.96E 03 alkB2 gene copies mL 1 . This study highlights the validity of the assay for the detection at single cell level of key functional genes involved in the biodegradation of n alkanes. 110 Published version In situ detection of alkB2 gene involved in Alcanivorax borkumensis SK2 T hydrocarbon biodegradation Matturro et al., 2016.pdf Articolo in rivista Macmillan, 0025 326X Marine pollution bulletin. Marine pollution bulletin. Mar. Pollut. Bull. Marine pollution bulletin. simone.cappello CAPPELLO SIMONE maria.genovese GENOVESE MARIA FRASCADORE EMANUELA simona.rossetti ROSSETTI SIMONA bruna.matturro MATTURRO BRUNA |