Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | Microbiome Dynamics of a Polychlorobiphenyl (PCB) Historically Contaminated Marine Sediment under Conditions Promoting Reductive Dechlorination |
| Abstract | The toxicity of polychlorinated biphenyls (PCB) can be efficiently reduced in contaminated marine sediments through the reductive dechlorination (RD) process lead by anaerobic organohalide bacteria. Although the process has been extensively investigated on PCB-spiked sediments, the knowledge on the identity and metabolic potential of PCB-dechlorinating microorganisms in real contaminated matrix is still limited. Aim of this study was to explore the composition and the dynamics of the microbial communities of the marine sediment collected from one of the largest Sites of National Interest (SIN) in Italy (Mar Piccolo, Taranto) under conditions promoting the PCBs RD. A long-term microcosm study revealed that autochthonous bacteria were able to sustain the PCB dechlorination at a high extent and the successive addition of an external fermentable organic substrate (lactate) caused the further depletion of the high-chlorinated PCBs (up to 70%). Next Generation Sequencing was used to describe the core microbiome of the marine sediment and to follow the changes caused by the treatments. OTUs affiliated to sulfur-oxidizing ?-proteobacteria, Sulfurovum, and Sulfurimonas, were predominant in the original sediment and increased up to 60% of total OTUs after lactate addition. Other OTUs detected in the sediment were affiliated to sulfate reducing (?-proteobacteria) and to organohalide respiring bacteria within Chloroflexi phylum mainly belonging to Dehalococcoidia class. Among others, Dehalococcoides mccartyi was enriched during the treatments even though the screening of the specific reductive dehalogenase genes revealed the occurrence of undescribed strains, which deserve further investigations. Overall, this study highlighted the potential of members of Dehalococcoidia class in reducing the contamination level of the marine sediment from Mar Piccolo with relevant implications on the selection of sustainable bioremediation strategies to clean-up the site. |
| Source | Frontiers in microbiology |
| Keywords | polychlorobiphenylsDehalococcoides mccartyimarine sedimentsreductive dechlorinationEpsilonproteobacteriaDehalococcoidianext generation sequencing (NGS)microbiome |
| Journal | Frontiers in microbiology |
| Editor | Frontiers Research Foundation,, Lausanne, Svizzera |
| Year | 2016 |
| Type | Articolo in rivista |
| DOI | 10.3389/fmicb.2016.01502 |
| Authors | Bruna Matturro, Carla Ubaldi and Simona Rossetti |
| Text | 359309 2016 10.3389/fmicb.2016.01502 ISI Web of Science WOS WOS 000383648200001 Scopus 2 s2.0 84994056189 polychlorobiphenyls Dehalococcoides mccartyi marine sediments reductive dechlorination Epsilonproteobacteria Dehalococcoidia next generation sequencing NGS microbiome Microbiome Dynamics of a Polychlorobiphenyl PCB Historically Contaminated Marine Sediment under Conditions Promoting Reductive Dechlorination Bruna Matturro, Carla Ubaldi and Simona Rossetti IRSA CNR, Water Research Institute National Research Council, Monterotondo, Italy ENEA, Technical Unit for Environmental Characterization, Prevention and Remediation, Centro Ricerche Casaccia, Rome, Italy The toxicity of polychlorinated biphenyls PCB can be efficiently reduced in contaminated marine sediments through the reductive dechlorination RD process lead by anaerobic organohalide bacteria. Although the process has been extensively investigated on PCB spiked sediments, the knowledge on the identity and metabolic potential of PCB dechlorinating microorganisms in real contaminated matrix is still limited. Aim of this study was to explore the composition and the dynamics of the microbial communities of the marine sediment collected from one of the largest Sites of National Interest SIN in Italy Mar Piccolo, Taranto under conditions promoting the PCBs RD. A long term microcosm study revealed that autochthonous bacteria were able to sustain the PCB dechlorination at a high extent and the successive addition of an external fermentable organic substrate lactate caused the further depletion of the high chlorinated PCBs up to 70% . Next Generation Sequencing was used to describe the core microbiome of the marine sediment and to follow the changes caused by the treatments. OTUs affiliated to sulfur oxidizing proteobacteria, Sulfurovum, and Sulfurimonas, were predominant in the original sediment and increased up to 60% of total OTUs after lactate addition. Other OTUs detected in the sediment were affiliated to sulfate reducing proteobacteria and to organohalide respiring bacteria within Chloroflexi phylum mainly belonging to Dehalococcoidia class. Among others, Dehalococcoides mccartyi was enriched during the treatments even though the screening of the specific reductive dehalogenase genes revealed the occurrence of undescribed strains, which deserve further investigations. Overall, this study highlighted the potential of members of Dehalococcoidia class in reducing the contamination level of the marine sediment from Mar Piccolo with relevant implications on the selection of sustainable bioremediation strategies to clean up the site. Published version https //www.frontiersin.org/articles/10.3389/fmicb.2016.01502/full Microbiome Dynamics of a Polychlorobiphenyl PCB Historically Contaminated Marine Sediment under Conditions Promoting Reductive Dechlorination Articolo fmicb 07 01502.pdf Articolo in rivista Frontiers Research Foundation, 1664 302X Frontiers in microbiology Frontiers in microbiology Front. microbiol. Frontiers in microbiology simona.rossetti ROSSETTI SIMONA bruna.matturro MATTURRO BRUNA |