Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | Metaproteomics Applied to Activated Sludge for Industrial Wastewater Treatment Revealed a Dominant Methylotrophic Metabolism of Hyphomicrobium zavarzinii |
| Abstract | In biological wastewater treatments, microbial populations of the so-called activated sludge work together in the abatement of pollutants. In this work, the metabolic behavior of the biomass of a lab-scale plant treating industrial pharmaceutical wastewater was investigated through a metaproteomic approach. The complete treatment process included a membrane biological reactor (MBR) coupled with an advanced oxidation process (AOP) for partial breakdown of non-biodegradable molecules. Proteins from biomass samples collected pre- and post-AOP application were investigated by two-dimensional gel electrophoresis (2DE), mass spectrometry (MS), and finally identified by database search. Results showed that most proteins remained constant between pre- and post-AOP. Methanol dehydrogenase (MDH) belonging to Hyphomicrobium zavarzinii appeared as the most constantly expressed protein in the studied consortium. Other identified proteins belonging to Hyphomicrobium spp. revealed a predominant methylotrophic metabolism, and H. zavarzinii appeared as a key actor in the studied microbial community. |
| Source | Microbial ecology 72 (1), pp. 9–13 |
| Keywords | MetaproteomicsActivated sludgeWastewaterHyphomicrobiumMethanol dehydrogenase |
| Journal | Microbial ecology |
| Editor | Springer-Verlag,, New York,, Stati Uniti d'America |
| Year | 2016 |
| Type | Articolo in rivista |
| DOI | 10.1007/s00248-016-0769-x |
| Authors | Carlo Salerno, Dirk Benndorf, Sabine Kluge, Luigi Leonardo Palese, Udo Reichl, Alfieri Pollice |
| Text | 354478 2016 10.1007/s00248 016 0769 x ISI Web of Science WOS WOS 000377998800003 Scopus 2 s2.0 84971632097 Metaproteomics Activated sludge Wastewater Hyphomicrobium Methanol dehydrogenase Metaproteomics Applied to Activated Sludge for Industrial Wastewater Treatment Revealed a Dominant Methylotrophic Metabolism of Hyphomicrobium zavarzinii Carlo Salerno, Dirk Benndorf, Sabine Kluge, Luigi Leonardo Palese, Udo Reichl, Alfieri Pollice IRSA Istituto di Ricerca Sulle Acque, Max Planck Institute for Dynamics of Complex Technical Systems of Magdeburg, Universita degli Studi Aldo Moro di Bari, Otto von Guericke University of Magdeburg In biological wastewater treatments, microbial populations of the so called activated sludge work together in the abatement of pollutants. In this work, the metabolic behavior of the biomass of a lab scale plant treating industrial pharmaceutical wastewater was investigated through a metaproteomic approach. The complete treatment process included a membrane biological reactor MBR coupled with an advanced oxidation process AOP for partial breakdown of non biodegradable molecules. Proteins from biomass samples collected pre and post AOP application were investigated by two dimensional gel electrophoresis 2DE , mass spectrometry MS , and finally identified by database search. Results showed that most proteins remained constant between pre and post AOP. Methanol dehydrogenase MDH belonging to Hyphomicrobium zavarzinii appeared as the most constantly expressed protein in the studied consortium. Other identified proteins belonging to Hyphomicrobium spp. revealed a predominant methylotrophic metabolism, and H. zavarzinii appeared as a key actor in the studied microbial community. 72 Published version http //link.springer.com/article/10.1007/s00248 016 0769 x Paper in formato PDF 2016 Salerno Metaproteomics .pdf Articolo in rivista Springer Verlag, 0095 3628 Microbial ecology Microbial ecology Microb Ecol Microbial ecology. alfieri.pollice POLLICE ALFIERI carlo.salerno SALERNO CARLO |