| Abstract | Crude oil is one of the most important natural assets for humankind, yet it is a major environmental pollutant, notably in marine environments. One of the largest crude oil polluted areas in the word is the semi-enclosed Mediterranean Sea, in which the metabolic potential of indigenous microbial populations towards the large-scale chronic pollution is yet to be defined, particularly in anaerobic and micro-aerophilic sites. Here, we provide an insight into the microbial metabolism in sediments from three chronically polluted marine sites along the coastline of Italy: the Priolo oil terminal/refinery site (near Siracuse, Sicily), harbour of Messina (Sicily) and shipwreck of MT Haven (near Genoa). Using shotgun metaproteomics and community metabolomics approaches, the presence of 651 microbial proteins and 4776 metabolite mass features have been detected in these three environments, revealing a high metabolic heterogeneity between the investigated sites. The proteomes displayed the prevalence of anaerobic metabolisms that were not directly related with petroleum biodegradation, indicating that in the absence of oxygen, biodegradation is significantly suppressed. This suppression was also suggested by examining the metabolome patterns. The proteome analysis further highlighted the metabolic coupling between methylotrophs and sulphate reducers in oxygen-depleted petroleum-polluted sediments. |
| Text | 349516 2015 10.1002/pmic.201400614 Scopus 2 s2.0 84944175369 Anaerobic Crude oil Hydrocarbonoclastic Mediterranean Sea Metabolomics Microbiology Metaproteomics and metabolomics analyses of chronically petroleum polluted sites reveal the importance of general anaerobic processes uncoupled with degradation Bargiela R.; Herbst F. A.; Martinez Martinez M.; Seifert J.; Rojo D.; Cappello S.; Genovese M.; Crisafi F.; Denaro R.; Chernikova T.N.; Barbas C.; von Bergen M.; Yakimov M.M.; Ferrer M.; Golyshin P.N. Consejo Superior de Investigaciones Cientificas CSIC , Institute of Catalysis, Madrid, Spain; Department of Proteomics, UFZ Helmholtz Centre for Environmental Research, Leipzig, Germany; Center for Microbial Communities, Department of Chemistry and Bioscience, Aalborg University, Aalborg, Denmark; Institute of Animal Science, Universitat Hohenheim, Stuttgart, Germany; Centro de Metabolomica y Bioanalisis CEMBIO , Facultad de Farmacia, Universidad CEU San Pablo, Madrid, Spain; Institute for Coastal Marine Environment, CNR, Messina, Italy; School of Biological Sciences, Bangor University, Gwynedd, United Kingdom; Department of Metabolomics, UFZ Helmholtz Centre for Environmental Research, Leipzig, Germany Crude oil is one of the most important natural assets for humankind, yet it is a major environmental pollutant, notably in marine environments. One of the largest crude oil polluted areas in the word is the semi enclosed Mediterranean Sea, in which the metabolic potential of indigenous microbial populations towards the large scale chronic pollution is yet to be defined, particularly in anaerobic and micro aerophilic sites. Here, we provide an insight into the microbial metabolism in sediments from three chronically polluted marine sites along the coastline of Italy the Priolo oil terminal/refinery site near Siracuse, Sicily , harbour of Messina Sicily and shipwreck of MT Haven near Genoa . Using shotgun metaproteomics and community metabolomics approaches, the presence of 651 microbial proteins and 4776 metabolite mass features have been detected in these three environments, revealing a high metabolic heterogeneity between the investigated sites. The proteomes displayed the prevalence of anaerobic metabolisms that were not directly related with petroleum biodegradation, indicating that in the absence of oxygen, biodegradation is significantly suppressed. This suppression was also suggested by examining the metabolome patterns. The proteome analysis further highlighted the metabolic coupling between methylotrophs and sulphate reducers in oxygen depleted petroleum polluted sediments. 15 Published version http //www.scopus.com/inward/record.url eid=2 s2.0 84944175369 partnerID=q2rCbXpz Bargiela et al 2015 Bargiela et al 2015.pdf Articolo in rivista Wiley VCH Verl. 1615 9853 Proteomics Weinh., Print Proteomics Weinh., Print Proteomics Weinh., Print Proteomics. Weinh., Print mikhail.iakimov IAKIMOV MIKHAIL renata.denaro DENARO RENATA francesca.crisafi CRISAFI FRANCESCA |
