Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | NaCl concentration in the medium modulates the secretion of active EmpA protease in Vibrio anguillarum at post-transcriptional level |
| Abstract | Aims: This study focused on the influence of different amounts of NaCl in the medium in Vibrio anguillarum EmpA protease production at both the transcriptional and post-transcriptional levels. Methods and Results: Vibrio anguillarum 975/I was cultivated in cM9 medium with varying concentrations of NaCl: 0·5, 1·5, 3·0%. EmpA protease was monitored in the supernatants by the skim milk test, azocasein assay and Western blot analysis. The empA gene expression was measured by real-time PCR. A mutant strain 975/I defective for the empA gene confirmed the specificity of the response for EmpA protease. Active protease production was induced by 0·5 and 1·5% NaCl-amended media; however, the strain cultivated in 3·0% NaCl was unable to secrete EmpA protease. The quantitative expression of the empA gene was very similar in all tested conditions. Conclusions: The NaCl concentration in the medium modulates the secretion of active EmpA protease in V. anguillarum at a post-transcriptional level. Significance and Impact of the Study: EmpA protease is one of the most important virulence factors in V. anguillarum. We demonstrated the influence of osmotic changes in the regulation of EmpA protease in the V. anguillarum 975/I strain. This finding has an important impact on the evaluation of factors determining the onset of disease in fish. |
| Source | Journal of applied microbiology (Online) 119 (6), pp. 1494–1501 |
| Keywords | Vibrio anguillarumempA gene expressionModulationProteaseSodium chloride |
| Journal | Journal of applied microbiology (Online) |
| Editor | Blackwell Science., Oxford, Regno Unito |
| Year | 2015 |
| Type | Articolo in rivista |
| DOI | 10.1111/jam.12957 |
| Authors | Crisafi F.; Denaro R.; Yakimov M.; Felice M.R.; Giuliano L.; Genovese L. |
| Text | 349508 2015 10.1111/jam.12957 Scopus 2 s2.0 84955192223 Vibrio anguillarum empA gene expression Modulation Protease Sodium chloride NaCl concentration in the medium modulates the secretion of active EmpA protease in Vibrio anguillarum at post transcriptional level Crisafi F.; Denaro R.; Yakimov M.; Felice M.R.; Giuliano L.; Genovese L. Institute for Coastal Marine Environment CNR, Messina, Italy; Department of Biological and Environmental Sciences, University of Messina, Messina, Italy Aims This study focused on the influence of different amounts of NaCl in the medium in Vibrio anguillarum EmpA protease production at both the transcriptional and post transcriptional levels. Methods and Results Vibrio anguillarum 975/I was cultivated in cM9 medium with varying concentrations of NaCl 0·5, 1·5, 3·0%. EmpA protease was monitored in the supernatants by the skim milk test, azocasein assay and Western blot analysis. The empA gene expression was measured by real time PCR. A mutant strain 975/I defective for the empA gene confirmed the specificity of the response for EmpA protease. Active protease production was induced by 0·5 and 1·5% NaCl amended media; however, the strain cultivated in 3·0% NaCl was unable to secrete EmpA protease. The quantitative expression of the empA gene was very similar in all tested conditions. Conclusions The NaCl concentration in the medium modulates the secretion of active EmpA protease in V. anguillarum at a post transcriptional level. Significance and Impact of the Study EmpA protease is one of the most important virulence factors in V. anguillarum. We demonstrated the influence of osmotic changes in the regulation of EmpA protease in the V. anguillarum 975/I strain. This finding has an important impact on the evaluation of factors determining the onset of disease in fish. 119 Published version http //www.scopus.com/inward/record.url eid=2 s2.0 84955192223 partnerID=q2rCbXpz Articolo in rivista Blackwell Science. 1365 2672 Journal of applied microbiology Online Journal of applied microbiology Online J. appl. microbiol. Online lucrezia.genovese GENOVESE LUCREZIA laura.giuliano GIULIANO LAURA mikhail.iakimov IAKIMOV MIKHAIL renata.denaro DENARO RENATA francesca.crisafi CRISAFI FRANCESCA |