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TitleNitrifying bacterial biomass and nitrification activity evaluated by FISH and an automatic on-line instrument at full-scale Fusina (Venice, Italy) WWTP
AbstractIn this study, monthly variations in biomass of ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB) were analysed over a 1-year period by fluorescence in situ hybridization (FISH) at the full-scale Fusina WWTP. The nitrification capacity of the plant was also monitored using periodic respirometric batch tests and by an automated on-line titrimetric instrument (TITrimetric Automated ANalyser). The percentage of nitrifying bacteria in the plant was the highest in summer and was in the range of 10-15%of the active biomass. The maximum nitrosation rate varied in the range 2.0-4.0 mg NH4 g-1 VSS h-1 (0.048- 0.096 kg TKNkg-1 VSSday-1): values obtained by laboratory measurements and the on-line instrument were similar and significantly correlated. The activity measurements provided a valuable tool for estimating the maximum total Kjeldahl nitrogen (TKN) loading possible at the plant and provided an early warning of whether the TKN was approaching its limiting value. The FISH analysis permitted determination of the nitrifying biomass present. The main operational parameter affecting both the population dynamics and the maximum nitrosation activity was mixed liquor volatile suspended solids (MLVSS) concentration and was negatively correlated with ammonia-oxidizing bacteria (AOB) (p=0.029) and (NOB) (p=0.01) abundances and positively correlated with maximum nitrosation rates (p=0.035). Increases in concentrations led to decreases in nitrifying bacteria abundance, but their nitrosation activity was higher. These results demonstrate the importance of MLVSS concentration as key factor in the development and activity of nitrifying communities in wastewater treatment plants (WWTPs). Operational data on VSS and sludge volume index (SVI) values are also presented on 11-year basis observations.
SourceEnvironmetal science and pollution research international (Internet)
KeywordsAOB . NOB . Nitrification . Nitrosation rate . Full-scaleWWTP . FISH (fluorescence in situ hybridization) . Respirometers . Automatic titrimetric systems
JournalEnvironmetal science and pollution research international (Internet)
EditorSpringer, Berlin, Germania
Year2015
TypeArticolo in rivista
AuthorsS. Badoer P. Miana S. Della Sala G. Marchiori V. Tandoi F. Di Pippo
Text349280 2015 AOB . NOB . Nitrification . Nitrosation rate . Full scaleWWTP . FISH fluorescence in situ hybridization . Respirometers . Automatic titrimetric systems Nitrifying bacterial biomass and nitrification activity evaluated by FISH and an automatic on line instrument at full scale Fusina Venice, Italy WWTP S. Badoer P. Miana S. Della Sala G. Marchiori V. Tandoi F. Di Pippo VERITAS, Venice, Italy Water Research Institute National Research Council IRSA CNR , Via Salaria km 29.300 CP10, 00015 Monterotondo, Roma, Italy Institute for Coastal Marine Environment National Research Council CNR IAMC , Localita Sa Mardini, Torregrande, Oristano, Italy In this study, monthly variations in biomass of ammonia oxidizing bacteria AOB and nitrite oxidizing bacteria NOB were analysed over a 1 year period by fluorescence in situ hybridization FISH at the full scale Fusina WWTP. The nitrification capacity of the plant was also monitored using periodic respirometric batch tests and by an automated on line titrimetric instrument TITrimetric Automated ANalyser . The percentage of nitrifying bacteria in the plant was the highest in summer and was in the range of 10 15%of the active biomass. The maximum nitrosation rate varied in the range 2.0 4.0 mg NH4 g 1 VSS h 1 0.048 0.096 kg TKNkg 1 VSSday 1 values obtained by laboratory measurements and the on line instrument were similar and significantly correlated. The activity measurements provided a valuable tool for estimating the maximum total Kjeldahl nitrogen TKN loading possible at the plant and provided an early warning of whether the TKN was approaching its limiting value. The FISH analysis permitted determination of the nitrifying biomass present. The main operational parameter affecting both the population dynamics and the maximum nitrosation activity was mixed liquor volatile suspended solids MLVSS concentration and was negatively correlated with ammonia oxidizing bacteria AOB p=0.029 and NOB p=0.01 abundances and positively correlated with maximum nitrosation rates p=0.035 . Increases in concentrations led to decreases in nitrifying bacteria abundance, but their nitrosation activity was higher. These results demonstrate the importance of MLVSS concentration as key factor in the development and activity of nitrifying communities in wastewater treatment plants WWTPs . Operational data on VSS and sludge volume index SVI values are also presented on 11 year basis observations. Published version Articolo in rivista Springer 1614 7499 Environmetal science and pollution research international Internet Environmetal science and pollution research international Internet Environ. sci. pollut. res. int. Internet Environmental science and pollution research international. Internet Environmental science and pollution research Internet Internet ESPR Internet Internet valter.tandoi TANDOI VALTER francesca.dipippo DI PIPPO FRANCESCA