Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | 1H-NMR analysis of water mobility in cultured phototrophic biofilms |
| Abstract | The present work reports on the first attempt to study water mobility in phototrophic biofilms, applying the 1HNMR relaxometry technique to closely monitored microbial communities grown in a microcosm under controlled ambient conditions. Longitudinal water proton relaxation times exhibited a bi-exponential behavior in all biofilm samples, indicating two types of water molecules with diverging dynamic properties, confined to different compartments of the biofilm. The fast-relaxing component can be attributed to water molecules tightly bound to the intracellular matrix, while the slow-relaxing component could reflect the behavior of water embedded in the biopolymer matrix, confined into matrix pores and channels. The results are discussed with respect to a possible key role of exopolysaccharides and uronic acids in water binding in phototrophic biofilms. |
| Source | Biofouling (Print) |
| Keywords | phototrophic biofilms; water binding; relaxation time; 1HNMR |
| Journal | Biofouling (Print) |
| Editor | Harwood Academic Publishers., New York, Svizzera |
| Year | 2011 |
| Type | Articolo in rivista |
| Authors | F. Di Pippo; A. Bohn; F. Cavalieri; P. Albertano |
| Text | 349276 2011 phototrophic biofilms; water binding; relaxation time; 1HNMR 1H NMR analysis of water mobility in cultured phototrophic biofilms F. Di Pippo; A. Bohn; F. Cavalieri; P. Albertano Department of Biology, University of Rome Tor Vergata , Rome b Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Oeiras, Portugal c Department of Chemical Sciences and Technologies, University of Rome Tor Vergata , Rome IRSA CNR The present work reports on the first attempt to study water mobility in phototrophic biofilms, applying the 1HNMR relaxometry technique to closely monitored microbial communities grown in a microcosm under controlled ambient conditions. Longitudinal water proton relaxation times exhibited a bi exponential behavior in all biofilm samples, indicating two types of water molecules with diverging dynamic properties, confined to different compartments of the biofilm. The fast relaxing component can be attributed to water molecules tightly bound to the intracellular matrix, while the slow relaxing component could reflect the behavior of water embedded in the biopolymer matrix, confined into matrix pores and channels. The results are discussed with respect to a possible key role of exopolysaccharides and uronic acids in water binding in phototrophic biofilms. Published version Articolo in rivista Harwood Academic Publishers. 0892 7014 Biofouling Print Biofouling Print Biofouling Print Biofouling. Print francesca.dipippo DI PIPPO FRANCESCA |