| Title | Polychlorinated biphenyl (PCB) anaerobic degradation in marine sediments: microcosm study and role of autochthonous microbial communities. |
| Abstract | Polychlorobiphenyl (PCB) biodegradation was followed for 1 year in microcosms containing marine sediments collected from Mar Piccolo (Taranto, Italy) chronically contaminated by this class of hazardous compounds. The microcosms were performed under strictly anaerobic conditions with or without the addition of Dehalococcoides mccartyi, the main microorganism known to degrade PCBs through the anaerobic reductive dechlorination process. Thirty PCB congeners were monitored during the experiments revealing that the biodegradation occurred in all microcosms with a decrease in hepta-, hexa-, and penta-chlorobiphenyls (CBs) and a parallel increase in low chlorinated PCBs (tri-CBs and tetra-CBs). The concentrations of the most representative congeners detected in the original sediment, such as 245-245-CB and 2345-245-CB, and of the mixture 2356-34-CB+234-245-CB, decreased by 32.5, 23.8, and 46.7 %, respectively, after only 70 days of anaerobic incubation without any bioaugmentation treatment. Additionally, the structure and population dynamics of the microbial key players involved in the biodegradative process and of the entire mixed microbial community were accurately defined by Catalyzed Reporter Deposition Fluorescence In Situ Hybridization (CARD-FISH) in both the original sediment and during the operation of the microcosm. The reductive dehalogenase genes of D. mccartyi, specifically involved in PCB dechlorination, were also quantified using real-time PCR (qPCR). Our results demonstrated that the autochthonous microbial community living in the marine sediment, including D. mccartyi (6.32E+06 16S rRNA gene copy numbers g(-1) sediment), was able to efficiently sustain the biodegradation of PCBs when controlled anaerobic conditions were imposed. |
| Source | Environmetal science and pollution research international (Internet), pp. 12613–12623 |
| Keywords | Polychlorinated biphenyls . Dehalococcoides mccartyi . Reductive dehalogenase genes . Bioremediation . Marine sediments . CARD-FISH . qPCR |
| Journal | Environmetal science and pollution research international (Internet) |
| Editor | Springer, Berlin, Germania |
| Year | 2016 |
| Type | Articolo in rivista |
| Authors | Matturro B, Ubaldi C, Grenni P, Barra Caracciolo A, Rossetti S |
| Text | 348629 2016 ISI Web of Science WOS 000378817300010 Polychlorinated biphenyls . Dehalococcoides mccartyi . Reductive dehalogenase genes . Bioremediation . Marine sediments . CARD FISH . qPCR Polychlorinated biphenyl PCB anaerobic degradation in marine sediments microcosm study and role of autochthonous microbial communities. Matturro B, Ubaldi C, Grenni P, Barra Caracciolo A, Rossetti S Water Research Institute, IRSA CNR, Via Salaria km 29, 300, Monterotondo, RM, Italy ENEA, Technical Unit for Environmental Characterization, Prevention and Remediation, UTPRA, C.R Casaccia, Rome, Italy Polychlorobiphenyl PCB biodegradation was followed for 1 year in microcosms containing marine sediments collected from Mar Piccolo Taranto, Italy chronically contaminated by this class of hazardous compounds. The microcosms were performed under strictly anaerobic conditions with or without the addition of Dehalococcoides mccartyi, the main microorganism known to degrade PCBs through the anaerobic reductive dechlorination process. Thirty PCB congeners were monitored during the experiments revealing that the biodegradation occurred in all microcosms with a decrease in hepta , hexa , and penta chlorobiphenyls CBs and a parallel increase in low chlorinated PCBs tri CBs and tetra CBs . The concentrations of the most representative congeners detected in the original sediment, such as 245 245 CB and 2345 245 CB, and of the mixture 2356 34 CB 234 245 CB, decreased by 32.5, 23.8, and 46.7 %, respectively, after only 70 days of anaerobic incubation without any bioaugmentation treatment. Additionally, the structure and population dynamics of the microbial key players involved in the biodegradative process and of the entire mixed microbial community were accurately defined by Catalyzed Reporter Deposition Fluorescence In Situ Hybridization CARD FISH in both the original sediment and during the operation of the microcosm. The reductive dehalogenase genes of D. mccartyi, specifically involved in PCB dechlorination, were also quantified using real time PCR qPCR . Our results demonstrated that the autochthonous microbial community living in the marine sediment, including D. mccartyi 6.32E 06 16S rRNA gene copy numbers g 1 sediment , was able to efficiently sustain the biodegradation of PCBs when controlled anaerobic conditions were imposed. Published version Polychlorinated biphenyl PCB anaerobic degradation in marine sediments microcosm study and role of autochthonous microbial communities Articolo Matturro et al., 2016_ESPR.pdf Articolo in rivista Springer 1614 7499 Environmetal science and pollution research international Internet Environmetal science and pollution research international Internet Environ. sci. pollut. res. int. Internet Environmental science and pollution research international. Internet Environmental science and pollution research Internet Internet ESPR Internet Internet simona.rossetti ROSSETTI SIMONA anna.barracaracciolo BARRA CARACCIOLO ANNA paola.grenni GRENNI PAOLA bruna.matturro MATTURRO BRUNA |
