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DatoValore
TitleStrategies for water quality assessment: A multiparametric analysis of microbiological changes in river waters
AbstractRiver waters are classified worldwide in relation to their chemical quality status, which is assessed by the presence of inorganic and organic pollutants. Meanwhile, microbiological quality evaluations are based mainly on the occurrence of pathogens and faecal contaminants, which are indirectly retrieved by standard cultivation methods. In this study, the structural dynamics of a riverine bacterioplankton community were investigated through a quantitative single-cell approach with the use of flow cytometry for the quantification of total, viable and active cells. Furthermore, catalyzed reporter deposition (CARD)-FISH was used for the evaluation of Proteobacteria and Cytophaga-Flavobacteria bacterial classes. The aim was to identify the major cytometric and phylogenetic cell groups that most representatively reflect the physical and chemical changes occurring in an upland-to-lowland transect along a human-impacted river (the Foglia River, Italy). The use of CARD-FISH to obtain cell abundance measurements of specific taxonomic clusters enabled different microbial propagation dynamics along the river transects to be identified. Furthermore, our results highlighted that the multiparametric approach with flow cytometry produces basic microbiological parameters in a reasonable time span that is consistent with the requirements for an early warning monitoring strategy with respect to integrated riverine water quality assessments.
SourceRiver research and applications (Print) 32 (3), pp. 490–500
KeywordsBacteriaCARD-FISHFlow cytometryRiverWater quality
JournalRiver research and applications (Print)
EditorJohn Wiley & Sons,, Chichester, West Sussex, UK, Regno Unito
Year2016
TypeArticolo in rivista
DOI10.1002/rra.2872
AuthorsBoi P.; Amalfitano S.; Manti A.; Semprucci F.; Sisti D.; Rocchi M.B.; Balsamo M.; Papa S.
Text348314 2016 10.1002/rra.2872 Scopus 2 s2.0 84959342391 ISI Web of Science WOS WOS 000372354700025 Bacteria CARD FISH Flow cytometry River Water quality Strategies for water quality assessment A multiparametric analysis of microbiological changes in river waters Boi P.; Amalfitano S.; Manti A.; Semprucci F.; Sisti D.; Rocchi M.B.; Balsamo M.; Papa S. Department of Earth, Life and Environmental Sciences University of Urbino Carlo Bo Urbino Italy; Water Research Institute IRSA CNR Rome Monterotondo Italy; Department of Biomolecular Sciences University of Urbino Carlo Bo Urbino Italy River waters are classified worldwide in relation to their chemical quality status, which is assessed by the presence of inorganic and organic pollutants. Meanwhile, microbiological quality evaluations are based mainly on the occurrence of pathogens and faecal contaminants, which are indirectly retrieved by standard cultivation methods. In this study, the structural dynamics of a riverine bacterioplankton community were investigated through a quantitative single cell approach with the use of flow cytometry for the quantification of total, viable and active cells. Furthermore, catalyzed reporter deposition CARD FISH was used for the evaluation of Proteobacteria and Cytophaga Flavobacteria bacterial classes. The aim was to identify the major cytometric and phylogenetic cell groups that most representatively reflect the physical and chemical changes occurring in an upland to lowland transect along a human impacted river the Foglia River, Italy . The use of CARD FISH to obtain cell abundance measurements of specific taxonomic clusters enabled different microbial propagation dynamics along the river transects to be identified. Furthermore, our results highlighted that the multiparametric approach with flow cytometry produces basic microbiological parameters in a reasonable time span that is consistent with the requirements for an early warning monitoring strategy with respect to integrated riverine water quality assessments. 32 Published version http //www.scopus.com/inward/record.url eid=2 s2.0 84922901613 partnerID=q2rCbXpz Strategies for water quality assessment A multiparametric analysis of microbiological changes in river waters Versione pubblicata Boi_2016_RRA_River waters.pdf Articolo in rivista John Wiley Sons, 1535 1459 River research and applications Print River research and applications Print River res. appl. Print River research and applications. Print River res. applic. Print stefano.amalfitano AMALFITANO STEFANO