Scheda di dettaglio – i prodotti della ricerca

DatoValore
TitleBiodegradation potentiality of psychrophilic bacterial strain Oleispira antarctica RB-8T
Abstracthe present study is focused on assessing the growth and hydrocarbon-degrading capability of the psychrophilic strain Oleispira antarctica RB-8T. This study considered six hydrocarbon mixtures that were tested for 22 days at two different cultivation temperatures (4 and 15 °C). During the incubation period, six sub-aliquots of each culture at different times were processed for total bacterial abundance and GC-FID (gas chromatography- flame ionization detection) hydrocarbon analysis. Results from DNA extraction and DAPI (4?,6-diamidino-2-phenylindole) staining showed a linear increase during the first 18 days of the experiment in almost all the substrates used; both techniques showed a good match, but the difference in values obtained was approximately one order of magnitude. GC-FID results revealed a substan- tial hydrocarbon degradation rate in almost all hydrocarbon sources and in particular at 15 °C rather than 4 °C (for commercial oil engine, oily waste, fuel jet, and crude oil). A more efficient degradation was observed in cultures grown with diesel and bilge water at 4 °C.
SourceMarine pollution bulletin.
KeywordsBioremediation Hydrocarbonoclastic bacteria Oil spill Oleispira antarctica Psychrophilic bacteria
JournalMarine pollution bulletin.
EditorMacmillan,, London, Regno Unito
Year2016
TypeArticolo in rivista
AuthorsG. Gentile a,?, M. Bonsignore a, S. Santisi a,b, M. Catalfamo a, L. Giuliano c, L. Genovese a, M.M. Yakimov a, R. Denaro a, M. Genovese a, S. Cappello a
Text347917 2016 Bioremediation Hydrocarbonoclastic bacteria Oil spill Oleispira antarctica Psychrophilic bacteria Biodegradation potentiality of psychrophilic bacterial strain Oleispira antarctica RB 8T G. Gentile a, , M. Bonsignore a, S. Santisi a,b, M. Catalfamo a, L. Giuliano c, L. Genovese a, M.M. Yakimov a, R. Denaro a, M. Genovese a, S. Cappello a IAMC CNR he present study is focused on assessing the growth and hydrocarbon degrading capability of the psychrophilic strain Oleispira antarctica RB 8T. This study considered six hydrocarbon mixtures that were tested for 22 days at two different cultivation temperatures 4 and 15 °C . During the incubation period, six sub aliquots of each culture at different times were processed for total bacterial abundance and GC FID gas chromatography flame ionization detection hydrocarbon analysis. Results from DNA extraction and DAPI 4 ,6 diamidino 2 phenylindole staining showed a linear increase during the first 18 days of the experiment in almost all the substrates used; both techniques showed a good match, but the difference in values obtained was approximately one order of magnitude. GC FID results revealed a substan tial hydrocarbon degradation rate in almost all hydrocarbon sources and in particular at 15 °C rather than 4 °C for commercial oil engine, oily waste, fuel jet, and crude oil . A more efficient degradation was observed in cultures grown with diesel and bilge water at 4 °C. Published version Biodegradation potentiality of psychrophilic bacterial strain Oleispira antarctica RB 8T Gentile et al., 2016 .pdf Articolo in rivista Macmillan, 0025 326X Marine pollution bulletin. Marine pollution bulletin. Mar. Pollut. Bull. Marine pollution bulletin. GENTILE GABRIELLA lucrezia.genovese GENOVESE LUCREZIA laura.giuliano GIULIANO LAURA renata.denaro DENARO RENATA simone.cappello CAPPELLO SIMONE maria.genovese GENOVESE MARIA maurizio.catalfamo CATALFAMO MAURIZIO