Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | Application of relative real-time PCR to detect differential expression of virulence genes in Vibrio anguillarum under standard and stressed growth conditions |
| Abstract | In this study, we aimed to understand whether abiotic factors affect the expression of virulence genes in Vibrio anguillarum. We observed the in vitro responses of two Mediterranean strains of V. anguillarum to temperature, NaCl and iron concentration changes. We monitored growth per- formance and gene transcription levels by compar- ing the results obtained under stressed conditions (temperatures of 5 °C, 15 °C and 37 °C; NaCl concentrations of 3% and 5%; and iron depletion and excess) with those obtained under standard growth conditions (25 °C, 1.5% NaCl and 0.6 lM of iron). The results showed that the strains respond differently. The strain 975/I was most strongly affected by conditions of 15 °C and iron depletion; these conditions induced increased transcription levels of empA, angR and fatA. Growth of the strain 17/I was inhibited at 15 °C and in iron depletion conditions; this strain also showed dramatic changes in the tran- scription levels of toxR and tonB2 under increased NaCl concentrations. These results demonstrate that environmental stress affects the expression of virulence genes in V. anguillarum that have impli- cations for the competitiveness, stress tolerance and the ability of V. anguillarum to cause infection. |
| Source | Journal of fish diseases (Online), pp. 1–12 |
| Keywords | gene expressionstress responseVibrio anguillarum. |
| Journal | Journal of fish diseases (Online) |
| Editor | Blackwell Science., Oxford, Regno Unito |
| Year | 2013 |
| Type | Articolo in rivista |
| Authors | F Crisafi, R Denaro, M Genovese, M Yakimov and L Genovese |
| Text | 347913 2013 gene expression stress response Vibrio anguillarum. Application of relative real time PCR to detect differential expression of virulence genes in Vibrio anguillarum under standard and stressed growth conditions F Crisafi, R Denaro, M Genovese, M Yakimov and L Genovese CNR IAMC In this study, we aimed to understand whether abiotic factors affect the expression of virulence genes in Vibrio anguillarum. We observed the in vitro responses of two Mediterranean strains of V. anguillarum to temperature, NaCl and iron concentration changes. We monitored growth per formance and gene transcription levels by compar ing the results obtained under stressed conditions temperatures of 5 °C, 15 °C and 37 °C; NaCl concentrations of 3% and 5%; and iron depletion and excess with those obtained under standard growth conditions 25 °C, 1.5% NaCl and 0.6 lM of iron . The results showed that the strains respond differently. The strain 975/I was most strongly affected by conditions of 15 °C and iron depletion; these conditions induced increased transcription levels of empA, angR and fatA. Growth of the strain 17/I was inhibited at 15 °C and in iron depletion conditions; this strain also showed dramatic changes in the tran scription levels of toxR and tonB2 under increased NaCl concentrations. These results demonstrate that environmental stress affects the expression of virulence genes in V. anguillarum that have impli cations for the competitiveness, stress tolerance and the ability of V. anguillarum to cause infection. Published version Application of relative real time PCR to detect differential expression of virulence genes in Vibrio anguillarum under standard and stressed growth Copia_di_Crisafi_et_al_2013.pdf Articolo in rivista Blackwell Science. 1365 2761 Journal of fish diseases Online Journal of fish diseases Online J. fish dis. Online francesca.crisafi CRISAFI FRANCESCA lucrezia.genovese GENOVESE LUCREZIA renata.denaro DENARO RENATA maria.genovese GENOVESE MARIA |