| Abstract | Regulatory
and risk assessment background and needs: In the context of the
European Union (EU) Water Framework Directive (WFD) it is
recognised that pharmaceuticals may pose a risk to the quality of
European water bodies. Three substances with pharmaceutical use are
included in the first so-called "watch list. However, monitoring of
steroidal estrogens may be difficult because of the detection limits of
most existing routine analytical methods and the high cost of high-end
analytical methods. Sensitive effect-based methods are of reducing the
current monitoring difficulties by measuring the estrogenic activity of
environmental samples in a cost-efficient way. Project description:The
project will focus on specific effect-based methods and best possible
analytical methods related to the watch list substances EE2, E2 and E1.
For this purpose, 20 l surface water and 20 wastewater samples across
Europe will be collected and analysed. Approximately 20 institutes or
agencies from 13 nations will be involved in the project. Detection
methods covered: Best possible chemical analysis (Joint Research
Centre (JRC), IT, and Federal Institute of Hydrology (BfG), DE) and
eight effect-based methods. Some of the effect-based methods applied
here are currently going through the OECD validation process or being
developed as ISO standards. Results and discussion: We mainly
discuss project characteristics and objectives in this abstract:
a) Promoting reliable screening methods to support the monitoring of
endocrine disrupting activity in wastewater and surface water
b) Harmonizing monitoring options across Europe c) Linking reliable
effect-based tools with regulatory needs d) Linking effect-based tools
with chemical analysis for estrogen monitoring e) Supporting national
and EU monitoring for endocrine disruptors Conclusions and expected
outcomes: Only a limited number of institutes in Europe currently have
the capacity to quantify the steroidal estrogens EE2 and E2 at their
suggested EQS levels. This could cause problems for the EU watch list
mechanism, where reliable exposure data are required. In this project we
will provide harmonised methods for sample collection, sample
extraction and data evaluation, and screening and risk assessment. This
activity will provide a proof-of-concept for integrated monitoring of
estrogenic substances and aims to bridge the gap between conventional
analytical and effect-based monitoring. |
| Authors | Robert Kase1, Inge Werner1, Henner Hollert2, Etienne Vermeirssen1, Sebastian Buchinger3, Peter Behnisch4, Barbora Jarosova5, Giulio Mariani6 , Bernd Gawlik6, Helen Clayton7 ,Olivier Perceval8, Selim Ait-Aissa9, Nicolas Creusot9, Georg Reifferscheid3, Thomas Ternes3, Christiane Heiss10,Carolina Di Paolo2, Thomas-Benjamin Seiler2, Petra Kunz11, Cornelia Kienle1, Valeria Dulio9, Sara Valsecchi12, Mario Carere13 |
| Text | 347749 2015 effect base analysis steroidal estrogens Water Framework Directive Effect based and chemical analytical monitoring for the steroidal estrogens An international project to cope with a monitoring challenge Robert Kase1, Inge Werner1, Henner Hollert2, Etienne Vermeirssen1, Sebastian Buchinger3, Peter Behnisch4, Barbora Jarosova5, Giulio Mariani6 , Bernd Gawlik6, Helen Clayton7 ,Olivier Perceval8, Selim Ait Aissa9, Nicolas Creusot9, Georg Reifferscheid3, Thomas Ternes3, Christiane Heiss10,Carolina Di Paolo2, Thomas Benjamin Seiler2, Petra Kunz11, Cornelia Kienle1, Valeria Dulio9, Sara Valsecchi12, Mario Carere13 Swiss Centre for Applied Ecotoxicology, EAWAG EPFL, Switzerland; 2RWTH Aachen University, Germany; 3Federal Institute of Hydrology, Germany 4Bio Detection Systems, Netherlands;5RECETOX, Czech Republic;6Joint Research Centre, European Commission; 7Directorate General Environment, European Commission; 8ONEMA, French National Agency for Water and Aquatic Environments, France; 9INERIS, France; 10Federal Environment Agency, Germany11Federal Office for the Environment, Switzerland; 12Water Research Institute, IRSA CNR, Italy;13Italian Institute of Health, Department Environment and Primary Prevention, Italy Regulatory and risk assessment background and needs In the context of the European Union EU Water Framework Directive WFD it is recognised that pharmaceuticals may pose a risk to the quality of European water bodies. Three substances with pharmaceutical use are included in the first so called watch list. However, monitoring of steroidal estrogens may be difficult because of the detection limits of most existing routine analytical methods and the high cost of high end analytical methods. Sensitive effect based methods are of reducing the current monitoring difficulties by measuring the estrogenic activity of environmental samples in a cost efficient way. Project description The project will focus on specific effect based methods and best possible analytical methods related to the watch list substances EE2, E2 and E1. For this purpose, 20 l surface water and 20 wastewater samples across Europe will be collected and analysed. Approximately 20 institutes or agencies from 13 nations will be involved in the project. Detection methods covered Best possible chemical analysis Joint Research Centre JRC , IT, and Federal Institute of Hydrology BfG , DE and eight effect based methods. Some of the effect based methods applied here are currently going through the OECD validation process or being developed as ISO standards. Results and discussion We mainly discuss project characteristics and objectives in this abstract a Promoting reliable screening methods to support the monitoring of endocrine disrupting activity in wastewater and surface water b Harmonizing monitoring options across Europe c Linking reliable effect based tools with regulatory needs d Linking effect based tools with chemical analysis for estrogen monitoring e Supporting national and EU monitoring for endocrine disruptors Conclusions and expected outcomes Only a limited number of institutes in Europe currently have the capacity to quantify the steroidal estrogens EE2 and E2 at their suggested EQS levels. This could cause problems for the EU watch list mechanism, where reliable exposure data are required. In this project we will provide harmonised methods for sample collection, sample extraction and data evaluation, and screening and risk assessment. This activity will provide a proof of concept for integrated monitoring of estrogenic substances and aims to bridge the gap between conventional analytical and effect based monitoring. Published version 25th Annual Meeting of SETAC Europe Environmental protection in a multi stressed world challenges for science, industry and regulators Barcelona, Catalonia, Spain 3 7 May 2015 Internazionale Contributo abstract poster abstracts_presentation_27162.pdf Poster saramaria.valsecchi VALSECCHI SARA MARIA |
