Title | Unveiling PHA-storing populations using molecular methods. |
Abstract | Enrichment of mixed microbial cultures (MMCs) in polyhydroxyalkanoate (PHA)-storing microorganisms must take place to develop a successful PHA production process. Moreover, throughout the operational period of a MMC system, the population needs to be checked in order to understand the changes in the performance that eventually occurred. For these reasons, it is necessary to monitor the population evolution, in order to identify the different groups of microorganisms and relate them with the storage capacity and kinetics of the MMC. Regarding this particular process, several culture-independent molecular techniques were already applied, with the use of hybridization techniques such fluorescence in situ hybridization and also PCR-based methods like denaturing gradient gel electrophoresis, terminal restriction fragment length polymorphism, pyrosequencing, and quantitative PCR standing out. This review intends, thus, to look at the molecular methods currently applied in monitoring the PHA-storing population evolution and how they can be combined with the evolutionary engineering step in order to optimize the overall process. |
Source | Applied microbiology and biotechnology 99 (24), pp. 10433–10446 |
Keywords | Mixed microbial culture; Polyhydroxyalkanoates; Ecology; Hybridization; PCR-based methods |
Journal | Applied microbiology and biotechnology |
Editor | Springer, Heidelberg ;, Germania |
Year | 2015 |
Type | Articolo in rivista |
DOI | 10.1007/s00253-015-7010-6 |
Authors | Queiros, Diogo; Lemos, Paulo C; Rossetti, Simona; Serafim, Luisa S |
Text | 341979 2015 10.1007/s00253 015 7010 6 ISI Web of Science WOS 26428237 Mixed microbial culture; Polyhydroxyalkanoates; Ecology; Hybridization; PCR based methods Unveiling PHA storing populations using molecular methods. Queiros, Diogo; Lemos, Paulo C; Rossetti, Simona; Serafim, Luisa S veiro Institute of Materials, Chemistry Department, University of Aveiro, Campus Universitario de Santiago, 3810 193 Aveiro, Portugal; Chemistry Department, Faculty of Science and Technology, University Nova of Lisbon, 2829 516 Caparica, Portugal; Water Research Institute, C.N.R., Via Salaria Km 29,300, 00015 Monterotondo, Italy Enrichment of mixed microbial cultures MMCs in polyhydroxyalkanoate PHA storing microorganisms must take place to develop a successful PHA production process. Moreover, throughout the operational period of a MMC system, the population needs to be checked in order to understand the changes in the performance that eventually occurred. For these reasons, it is necessary to monitor the population evolution, in order to identify the different groups of microorganisms and relate them with the storage capacity and kinetics of the MMC. Regarding this particular process, several culture independent molecular techniques were already applied, with the use of hybridization techniques such fluorescence in situ hybridization and also PCR based methods like denaturing gradient gel electrophoresis, terminal restriction fragment length polymorphism, pyrosequencing, and quantitative PCR standing out. This review intends, thus, to look at the molecular methods currently applied in monitoring the PHA storing population evolution and how they can be combined with the evolutionary engineering step in order to optimize the overall process. 99 Published version Articolo in rivista Springer 0175 7598 Applied microbiology and biotechnology Applied microbiology and biotechnology Appl. microbiol. biotechnol. Applied microbiology and biotechnology. Applied microbiology and biotechnology Print simona.rossetti ROSSETTI SIMONA TA.P07.017.001 Caratterizzazione di biomasse per l ottimizzazione dei processi biologici |