| Title | Linking multiple stressors to microbial community structure for water quality assessment |
| Abstract | Freshwater quality assessment in Europe is established under the Water Frame Directive (WFD) based on measurements of chemical pollutants in sediments and water and biota (chemical status) and on the ecological indicators such as nutrients, temperature, species compositions etc. (ecological status). However in the current strategy a link between chemical and ecological status is missing. No biological indicators are foreseen to integrate the different anthropogenic pressures including chemical mixture, nutrients, temperature changes in order to provide a global scenario on the ecosystem and therefore on the water quality. In this context, MicroCokit FP7-PEOPLE-2012-IAPP project is focused on the identification of microbial community based indicators for monitoring and evaluating the complexity of multiple stressors (e.g. chemical pollutants, temperature, nutrients etc.) relevant to guide preventive and remediation actions by the water authorities. For this purpose the river Tiber has been chosen as a pilot case study and sampling sites were selected based on different anthropogenic pressures which they are exposed to. Water samples are being analyzed for both microbiological (FISH analysis and Metagenomic) and chemical analysis (organic and inorganic compounds, including emerging pollutants). Within the 4-years project, two campaigns are contemplated per year (Autumn and Spring). We report preliminary results of the chemical analyses (inorganic elements, DOC, PAHs, organochlorine, triazine, chloroacetamide pesticides, perfluorinated compounds, pharmaceuticals, etc.) and epifluorescence direct microscopy analyses (total microbial abundance and phylogenetic community characterization by FISH) regarding the first two campaigns. The preliminary results suggest that the microbial community structure reflects both natural environmental variations (river course and sesonality) and anthropogenic pressures. The same water samples are currently being processed for metagenomic analysis in order to obtain a broader description of the microbial community to be compared with the overall chemical results. |
| Source | Environmental protection in a multi-stressed world: challenges for science, industry and regulators, SETAC Europe 25th Annual Meeting, Barcellona, Spain, 3-7 May 2015 |
| Keywords | water qualitymicroorganismsanthropogenic pressurebioindicators |
| Year | 2015 |
| Type | Abstract in atti di convegno |
| Authors | Barra Caracciolo A, Ferrero VEV, Grenni P, Di Lenola M, Ademollo N, Patrolecco L, Loos R, Tavazzi S, Pinto Grande J, Saccà ML, Lettieri T |
| Text | 340869 2015 water quality microorganisms anthropogenic pressure bioindicators Linking multiple stressors to microbial community structure for water quality assessment Barra Caracciolo A, Ferrero VEV, Grenni P, Di Lenola M, Ademollo N, Patrolecco L, Loos R, Tavazzi S, Pinto Grande J, Sacca ML, Lettieri T Barra Caracciolo A, Grenni P, Di Lenola M, Ademollo N, Patrolecco L, Sacca ML IRSA CNR Ferrero VEV, Loos R, Tavazzi S, Pinto Grande J, Lettieri T JRC, Ispra SETAC EUROPE Published version https //www.google.it/url sa=t rct=j q= esrc=s source=web cd=1 ved=0ahUKEwjQosqWn5XLAhUPhhoKHSDNCRIQFggcMAA url=https%3A%2F%2Fwww.setac.org%2Fresource%2Fresmgr%2FAbstract_Books%2FSETAC Barcelona abstracts.pdf usg=AFQjCNFiKq3 eo7_qu aexk5Eo6 lQN14g sig2=X0Ah2hE3jjpYCPnz8Sh 5w bvm=bv.115339255,d.d2s cad=rja ISSN 2309 8031 Environmental protection in a multi stressed world challenges for science, industry and regulators, SETAC Europe 25th Annual Meeting Barcellona, Spain 3 7 May 2015 Internazionale Contributo Freshwater quality assessment in Europe is established under the Water Frame Directive WFD based on measurements of chemical pollutants in sediments and water and biota chemical status and on the ecological indicators such as nutrients, temperature, species compositions etc. ecological status . However in the current strategy a link between chemical and ecological status is missing. No biological indicators are foreseen to integrate the different anthropogenic pressures including chemical mixture, nutrients, temperature changes in order to provide a global scenario on the ecosystem and therefore on the water quality. In this context, MicroCokit FP7 PEOPLE 2012 IAPP project is focused on the identification of microbial community based indicators for monitoring and evaluating the complexity of multiple stressors e.g. chemical pollutants, temperature, nutrients etc. relevant to guide preventive and remediation actions by the water authorities. For this purpose the river Tiber has been chosen as a pilot case study and sampling sites were selected based on different anthropogenic pressures which they are exposed to. Water samples are being analyzed for both microbiological FISH analysis and Metagenomic and chemical analysis organic and inorganic compounds, including emerging pollutants . Within the 4 years project, two campaigns are contemplated per year Autumn and Spring . We report preliminary results of the chemical analyses inorganic elements, DOC, PAHs, organochlorine, triazine, chloroacetamide pesticides, perfluorinated compounds, pharmaceuticals, etc. and epifluorescence direct microscopy analyses total microbial abundance and phylogenetic community characterization by FISH regarding the first two campaigns. The preliminary results suggest that the microbial community structure reflects both natural environmental variations river course and sesonality and anthropogenic pressures. The same water samples are currently being processed for metagenomic analysis in order to obtain a broader description of the microbial community to be compared with the overall chemical results. Abstract Book SETAC_Barcelona_abstracts_1 0.pdf Abstract in atti di convegno luisa.patrolecco PATROLECCO LUISA anna.barracaracciolo BARRA CARACCIOLO ANNA paola.grenni GRENNI PAOLA nicoletta.ademollo ADEMOLLO NICOLETTA MICROCOKIT Microbial Community based sequencing analysis linked to anthropogenic pressures MicroCoKit to address the water quality TA.P04.005.011 Vulnerabilita degli ecosistemi delle acque sotterranee e attenuazione naturale degli inquinanti nel suolo e nel sottosuolo |
