| Abstract | 1. Freshwater mussels of the family Unionidae are one of the most threatened groups worldwide and have
suffered severe decline over recent decades. Although the freshwater duck mussel, Anodonta anatina (Linnaeus,
1758), is still widespread, this species has shown evidence of recent declines and is already protected in some
European countries.
2. Informed conservation efforts must take into account patterns in genetic diversity and phylogeography. In the
present study, 20 newly developed polymorphic loci were described and tested in seven populations of A. anatina,
belonging to three previously detected divergent mtDNA lineages. The genetic diversity patterns, within and
among A. anatina populations, were evaluated to test their congruence with those lineages.
3. A high genetic differentiation (FST) was found among all populations, with the exception of those in Central
Europe (Germany) and the UK, which were not strongly structured.
4. The present study confirms the division of the species into three evolutionarily significant units corresponding
to the three previously detected mtDNA lineages, which should be managed independently. Furthermore, owing
to the high differentiation among southern European populations, the establishment of distinct management
units for the Guadalquivir, Guadiana and Douro populations in the Iberian Peninsula is also proposed. |
| Authors | MANUEL LOPES-LIMAa,b,*, RONALDO SOUSAa,c, AMÍLCAR TEIXEIRAd, SIMONE VARANDASe, NICOLETTA RICCARDIf, DAVID C. ALDRIDGEg and ELSA FROUFEa |
| Text | 331805 2016 10.1002/aqc.2575 Scopus 2 s2.0 84931843591 ISI Web of Science WOS WOS 000373957200008 Anodonta anatina; anodontini; genetic diversity; microsatellites; Unionidae; conservation genetics Newly developed microsatellite markers for the pan European duck mussel, Anodonta anatina revisiting the main mitochondrial lineages MANUEL LOPES LIMAa,b, , RONALDO SOUSAa,c, AMÍLCAR TEIXEIRAd, SIMONE VARANDASe, NICOLETTA RICCARDIf, DAVID C. ALDRIDGEg and ELSA FROUFEa aInterdisciplinary Centre of Marine and Environmental Research CIIMAR/CIMAR , University of Porto, Porto, Portugal bIUCN/SSC Mollusc Specialist Group, c/o IUCN, Cambridge, UK cCBMA Centre of Molecular and Environmental Biology, Department of Biology, University of Minho, Braga, Portugal dCIMO/ESA/IPB Mountain Research Centre, School of Agriculture, Polytechnic Institute of Bragança, Bragança, Portugal eCITAB/UTAD Forestry Department, Centre for Research and Technology of Agro Environment and Biological Sciences, University of Tras os Montes and Alto Douro, Vila Real, Portugal fCNR Institute for Ecosystems Studies, Verbania Pallanza VB , Italy gUniversity of Cambridge, Aquatic Ecology Group, Department of Zoology, Cambridge, UK 1. Freshwater mussels of the family Unionidae are one of the most threatened groups worldwide and have suffered severe decline over recent decades. Although the freshwater duck mussel, Anodonta anatina Linnaeus, 1758 , is still widespread, this species has shown evidence of recent declines and is already protected in some European countries. 2. Informed conservation efforts must take into account patterns in genetic diversity and phylogeography. In the present study, 20 newly developed polymorphic loci were described and tested in seven populations of A. anatina, belonging to three previously detected divergent mtDNA lineages. The genetic diversity patterns, within and among A. anatina populations, were evaluated to test their congruence with those lineages. 3. A high genetic differentiation FST was found among all populations, with the exception of those in Central Europe Germany and the UK, which were not strongly structured. 4. The present study confirms the division of the species into three evolutionarily significant units corresponding to the three previously detected mtDNA lineages, which should be managed independently. Furthermore, owing to the high differentiation among southern European populations, the establishment of distinct management units for the Guadalquivir, Guadiana and Douro populations in the Iberian Peninsula is also proposed. 26 Published version http //onlinelibrary.wiley.com/journal/10.1002/ ISSN 1099 0755 27/04/2015 Newly developed microsatellite markers for the pan European duck mussel, Anodonta anatina revisiting the main mitochondrial lineages Lopes_Lima_et_al_2015_Aquatic_Conservation_Marine_and_Freshwater_Ecosystems.pdf Articolo in rivista John Wiley Sons, Ltd. 1099 0755 Aquatic conservation Online Aquatic conservation Online Aquat. conserv. Online Aquatic conservation Online Marine and freshwater ecosystems Online nicolettarita.riccardi RICCARDI NICOLETTA RITA |
