| Text | 331542 2015 10.1016/j.ecolind.2015.04.021 Scopus 2 s2.0 84929493342 Acute hatching test Acute mortality test Artemia Intercalibration activities Long term mortality test Intercalibration of ecotoxicity testing protocols with Artemia franciscana Manfra L.; Savorelli F.; Di Lorenzo B.; Libralato G.; Comin S.; Conti D.; Floris B.; Francese M.; Gallo M.L.; Gartner I.; Guida M.; Leoni T.; Marino G.; Martelli F.; Palazzi D.; Prato E.; Righini P.; Rossi E.; Volpi Ghirardini A.; Migliore L. Italian Institute for Environmental Protection and Research, Istituto Superiore per la Protezione e la Ricerca Ambientale, Via Vitaliano Brancati, 60, Rome, 00144, Italy; Institute for the Coastal Marine Environment, National Research Council CNR IAMC , Via Roma 3, Taranto, 74100, Italy; Regional Agency for Environmental Protection, Via Bologna 534, Ferrara, 44124, Italy; Department of Environmental Sciences, Informatics and Statistics, University Ca Foscari Venice, Campo della Celestia, 2737/b, Venice, I 30122, Italy; Institute of Marine Sciences, National Research Council CNR ISMAR , Arsenale Tesa 104, Castello 2737/F, Venice, I 30122, Italy; Regional Agency for Environmental Protection, Via Lissa, 6, Venice, 30174, Italy; Regional Agency for Environmental Protection, Viale Ciusa 6, Cagliari, Italy; Shoreline, Area Science Park Padriciano 99, Trieste, 34149, Italy; Regional Agency for Environmental Protection, Via Don Bosco 4/F, Naples, 80141, Italy; Regional Agency for Environmental Protection, Via Adige 12, Piombino, 57025, Italy; Department of Biology, University of Naples Federico II, via Mezzocannone 16, Naples, 80134, Italy; Regional Agency for Environmental Protection, Via Federico II 41 loc. Villa Potenza, Macerata, 62010, Italy; Regional Agency for Environmental Protection, Via Nairobi 4, Palermo, 90100, Italy; Regional Agency for Environmental Protection, Via Fiume 35, Grosseto, 58100, Italy; Regional Agency for Environmental Protection, Via Marradi 114, Livorno, 57126, Italy; Regional Agency for Environmental Protection, via Fontevivo 21, La Spezia, 19125, Italy; Department of Biology, University Tor Vergata, Via della Ricerca Scientifica, Rome, 00133, Italy The brine shrimp, Artemia spp., is widely used in ecotoxicology as a target biological model. Although several protocols were available in the early 1980s, only the 24 h acute mortality toxicity test was evaluated in a European intercalibration exercise during that period. Nevertheless, documentation of standard methods serving to provide specifications, guidelines or detailed characteristics of the 24 h protocol is still unavailable. This paper present the results of an intercalibration study of three toxicity testing protocols using Artemia franciscana a the 24 h static acute mortality test, b the 48 h static hatching test and c the 14 d static renewal long term mortality test. A first tier of experiments was conducted by a reference laboratory, which investigated the repeatability of the three methods. The feasibility and reproducibility of these protocols were then investigated by an intercomparison exercise involving 11 participants for the acute mortality test, seven for the acute hatching test and nine for the long term mortality test. Protocols were tested on reference toxicants copper sulphate pentahydrate and sodium dodecyl sulphate . The coefficients of variation were <20% and <50% for intra and interlaboratory activities, respectively. These results encourage the standardization of the proposed methods and their use as regulatory procedures. 57 Published version http //www.scopus.com/inward/record.url eid=2 s2.0 84929493342 partnerID=q2rCbXpz Intercalibration of ecotoxicity testing protocols with Artemia franciscana 1_s2_0_S1470160X15001995_main.pdf Articolo in rivista Elsevier Science Ireland. 1470 160X Ecological indicators Ecological indicators Ecological indicators. VOLPI GHIRARDINI ANNAMARIA LIBRALATO GIOVANNI MANFRA LOREDANA ermelinda.prato PRATO ERMELINDA |
